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Open AccessArticle

Phosphoinositide Profile of the Mouse Retina

1
Department of Ophthalmology, Duke University, Durham, NC 27710, USA
2
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
3
Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
4
Department of Ophthalmology, University of Florida, Gainesville, FL 32610, USA
5
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32610, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(6), 1417; https://doi.org/10.3390/cells9061417
Received: 7 April 2020 / Revised: 18 May 2020 / Accepted: 4 June 2020 / Published: 7 June 2020
(This article belongs to the Section Cells of the Nervous System)
Phosphoinositides are known to play multiple roles in eukaryotic cells. Although dysregulation of phosphoinositide metabolism in the retina has been reported to cause visual dysfunction in animal models and human patients, our understanding of the phosphoinositide composition of the retina is limited. Here, we report a characterization of the phosphoinositide profile of the mouse retina and an analysis of the subcellular localization of major phosphorylated phosphoinositide forms in light-sensitive photoreceptor neurons. Using chromatography of deacylated phosphatidylinositol headgroups, we established PI(4,5)P2 and PI(4)P as two major phosphorylated phosphoinositides in the retina. Using high-resolution mass spectrometry, we revealed 18:0/20:4 and 16:0/20:4 as major fatty-acyl chains of retinal phosphoinositides. Finally, analysis of fluorescent phosphoinositide sensors in rod photoreceptors demonstrated distinct subcellular distribution patterns of major phosphoinositides. The PI(4,5)P2 reporter was enriched in the inner segments and synapses, but was barely detected in the light-sensitive outer segments. The PI(4)P reporter was mostly found in the outer and inner segments and the areas around nuclei, but to a lesser degree in the synaptic region. These findings provide support for future mechanistic studies defining the biological significance of major mono- (PI(4)P) and bisphosphate (PI(4,5)P2) phosphatidylinositols in photoreceptor biology and retinal health. View Full-Text
Keywords: retina; phosphatidylinositol phosphate; phosphatidylinositol bisphosphate; photoreceptor retina; phosphatidylinositol phosphate; phosphatidylinositol bisphosphate; photoreceptor
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MDPI and ACS Style

Finkelstein, S.; Gospe, S.M., III; Schuhmann, K.; Shevchenko, A.; Arshavsky, V.Y.; Lobanova, E.S. Phosphoinositide Profile of the Mouse Retina. Cells 2020, 9, 1417. https://doi.org/10.3390/cells9061417

AMA Style

Finkelstein S, Gospe SM III, Schuhmann K, Shevchenko A, Arshavsky VY, Lobanova ES. Phosphoinositide Profile of the Mouse Retina. Cells. 2020; 9(6):1417. https://doi.org/10.3390/cells9061417

Chicago/Turabian Style

Finkelstein, Stella; Gospe, Sidney M., III; Schuhmann, Kai; Shevchenko, Andrej; Arshavsky, Vadim Y.; Lobanova, Ekaterina S. 2020. "Phosphoinositide Profile of the Mouse Retina" Cells 9, no. 6: 1417. https://doi.org/10.3390/cells9061417

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