Protein Phosphatases Type 2C Group A Interact with and Regulate the Stability of ACC Synthase 7 in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Growth Conditions and Treatments
2.2. Plant Transformation
2.3. Vector Constructs
2.4. Microscopy Studies in Arabidopsis Protoplasts
2.5. Ethylene Measurements
2.6. Recombinant Protein Expression and Purification
2.7. Pull-Down Assay
2.8. Cell-Free Degradation Assay
2.9. Immunoblotting
2.10. ACS7-ABI1 Protein Complex Modeling
3. Results
3.1. ACS7 and Group A PP2Cs Are Localized in both Nucleus and Cytoplasm
3.2. ACS7 Interacts with Group A Protein Phosphatases Type 2C
3.3. Modeling of the ACS7–ABI1 PP2C Complex
3.4. ABI1 W300 Is Important for Interaction with ACS7
3.5. ABI1 Regulates ACS7 Turnover
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Marczak, M.; Cieśla, A.; Janicki, M.; Kasprowicz-Maluśki, A.; Kubiak, P.; Ludwików, A. Protein Phosphatases Type 2C Group A Interact with and Regulate the Stability of ACC Synthase 7 in Arabidopsis. Cells 2020, 9, 978. https://doi.org/10.3390/cells9040978
Marczak M, Cieśla A, Janicki M, Kasprowicz-Maluśki A, Kubiak P, Ludwików A. Protein Phosphatases Type 2C Group A Interact with and Regulate the Stability of ACC Synthase 7 in Arabidopsis. Cells. 2020; 9(4):978. https://doi.org/10.3390/cells9040978
Chicago/Turabian StyleMarczak, Małgorzata, Agata Cieśla, Maciej Janicki, Anna Kasprowicz-Maluśki, Piotr Kubiak, and Agnieszka Ludwików. 2020. "Protein Phosphatases Type 2C Group A Interact with and Regulate the Stability of ACC Synthase 7 in Arabidopsis" Cells 9, no. 4: 978. https://doi.org/10.3390/cells9040978