Next Article in Journal
Aldosterone Decreases Vasopressin-Stimulated Water Reabsorption in Rat Inner Medullary Collecting Ducts
Previous Article in Journal
Human Liver Spheroids as a Model to Study Aetiology and Treatment of Hepatic Fibrosis
Open AccessArticle

Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine

1
Abiel s.r.l, c/o University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy
2
Institute for Biomedical Research and Innovation-National Research Council (IRIB-CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy
3
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies [STEBICEF], University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy
*
Author to whom correspondence should be addressed.
Cells 2020, 9(4), 962; https://doi.org/10.3390/cells9040962
Received: 31 March 2020 / Revised: 7 April 2020 / Accepted: 7 April 2020 / Published: 14 April 2020
Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone is required for effective cartilage digestion, resulting in an improvement in the yield of viable cells. The extracted chondrocytes proved able to grow and activate differentiation/dedifferentiation programs, as assessed by morphological and gene expression analyses. View Full-Text
Keywords: nasal chondrocytes; tissue dissociation; collagenases; gene expression profiles; cell transplantation nasal chondrocytes; tissue dissociation; collagenases; gene expression profiles; cell transplantation
Show Figures

Figure 1

MDPI and ACS Style

Salamone, M.; Rigogliuso, S.; Nicosia, A.; Tagliavia, M.; Campora, S.; Cinà, P.; Bruno, C.; Ghersi, G. Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine. Cells 2020, 9, 962.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop