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Article

Ca2+–Calmodulin Dependent Wound Repair in Dictyostelium Cell Membrane

1
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8511, Japan
2
Institute of Food and Radiation Biology, AERE, Bangladesh Atomic Energy Commission, Savar, Dhaka 3787, Bangladesh
3
Rajshahi Diabetic Association General Hospital, Luxmipur, Jhautala, Rajshahi 6000, Bangladesh
4
Department of Molecular Medicine and Biochemistry, Akita University Graduate School of Medicine, Akita 010-8543, Japan
*
Author to whom correspondence should be addressed.
Cells 2020, 9(4), 1058; https://doi.org/10.3390/cells9041058
Received: 2 April 2020 / Revised: 18 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020
(This article belongs to the Special Issue Molecular Mechanism for Wound Repair of Cell Membrane)
Wound repair of cell membrane is a vital physiological phenomenon. We examined wound repair in Dictyostelium cells by using a laserporation, which we recently invented. We examined the influx of fluorescent dyes from the external medium and monitored the cytosolic Ca2+ after wounding. The influx of Ca2+ through the wound pore was essential for wound repair. Annexin and ESCRT components accumulated at the wound site upon wounding as previously described in animal cells, but these were not essential for wound repair in Dictyostelium cells. We discovered that calmodulin accumulated at the wound site upon wounding, which was essential for wound repair. The membrane accumulated at the wound site to plug the wound pore by two-steps, depending on Ca2+ influx and calmodulin. From several lines of evidence, the membrane plug was derived from de novo generated vesicles at the wound site. Actin filaments also accumulated at the wound site, depending on Ca2+ influx and calmodulin. Actin accumulation was essential for wound repair, but microtubules were not essential. A molecular mechanism of wound repair will be discussed. View Full-Text
Keywords: actin; calcium ion; calmodulin; cell membrane; Dictyostelium; wound repair actin; calcium ion; calmodulin; cell membrane; Dictyostelium; wound repair
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MDPI and ACS Style

Talukder, M.S.U.; Pervin, M.S.; Tanvir, M.I.O.; Fujimoto, K.; Tanaka, M.; Itoh, G.; Yumura, S. Ca2+–Calmodulin Dependent Wound Repair in Dictyostelium Cell Membrane. Cells 2020, 9, 1058. https://doi.org/10.3390/cells9041058

AMA Style

Talukder MSU, Pervin MS, Tanvir MIO, Fujimoto K, Tanaka M, Itoh G, Yumura S. Ca2+–Calmodulin Dependent Wound Repair in Dictyostelium Cell Membrane. Cells. 2020; 9(4):1058. https://doi.org/10.3390/cells9041058

Chicago/Turabian Style

Talukder, Md. S.U., Mst. S. Pervin, Md. I.O. Tanvir, Koushiro Fujimoto, Masahito Tanaka, Go Itoh, and Shigehiko Yumura. 2020. "Ca2+–Calmodulin Dependent Wound Repair in Dictyostelium Cell Membrane" Cells 9, no. 4: 1058. https://doi.org/10.3390/cells9041058

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