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Purity Determines the Effect of Extracellular Vesicles Derived from Mesenchymal Stromal Cells
Open AccessArticle

Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model

1
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil
2
National Institute of Science and Technology for Regenerative Medicine-REGENERA, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil
3
Department of Biomedical Sciences, University of Padova, 35131 Padua, Italy
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National Center for Structural Biology and Bioimaging/CENABIO, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil
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Department of Medical Sciences, Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy
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Department of Pediatrics’ Section of Pediatric Nephrology, Tulane University School of Medicine, New Orleans, LA 70112, USA
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Department of Molecular Biotechnology and Health Sciences, University of Torino, 10126 Turin, Italy
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National Institute of Science and Technology of Structural Biology and Bioimaging-INBEB, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil
9
Graduate Program of Translational Biomedicine/BIOTRANS, Grande Rio University, 25071-202 Duque de Caxias, Brazil
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(2), 453; https://doi.org/10.3390/cells9020453
Received: 24 November 2019 / Revised: 16 January 2020 / Accepted: 12 February 2020 / Published: 17 February 2020
(This article belongs to the Special Issue Therapeutic Applications of Extracellular Vesicles)
Induced pluripotent stem cells (iPSC) have been the focus of several studies due to their wide range of application, including in cellular therapy. The use of iPSC in regenerative medicine is limited by their tumorigenic potential. Extracellular vesicles (EV) derived from stem cells have been shown to support renal recovery after injury. However, no investigation has explored the potential of iPSC-EV in the treatment of kidney diseases. To evaluate this potential, we submitted renal tubule cells to hypoxia-reoxygenation injury, and we analyzed cell death rate and changes in functional mitochondria mass. An in vivo model of ischemia-reperfusion injury was used to evaluate morphological and functional alterations. Gene array profile was applied to investigate the mechanism involved in iPSC-EV effects. In addition, EV derived from adipose mesenchymal cells (ASC-EV) were also used to compare the potential of iPSC-EV in support of tissue recovery. The results showed that iPSC-EV were capable of reducing cell death and inflammatory response with similar efficacy than ASC-EV. Moreover, iPSC-EV protected functional mitochondria and regulated several genes associated with oxidative stress. Taken together, these results show that iPSC can be an alternative source of EV in the treatment of different aspects of kidney disease. View Full-Text
Keywords: iPSC; extracellular vesicles; AKI; mitochondria; ROS; kidney iPSC; extracellular vesicles; AKI; mitochondria; ROS; kidney
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Collino, F.; Lopes, J.A.; Tapparo, M.; Tortelote, G.G.; Kasai-Brunswick, T.H.; Lopes, G.M.; Almeida, D.B.; Skovronova, R.; Wendt, C.H.C.; Miranda, K.R.; Bussolati, B.; Vieyra, A.; Lindoso, R.S. Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model. Cells 2020, 9, 453.

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