The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

Randelli, Filippo; Sartori, Patrizia; Carlomagno, Cristiano; Bedoni, Marzia; Menon, Alessandra; Vezzoli, Elena; Sommariva, Michele; Gagliano, Nicoletta

doi:10.3390/cells9122641

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The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

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Hip Department (CAD) Gaetano Pini—CTO Orthopedic Institute, Università degli Studi di Milano, Piazza Cardinal Ferrari 1, 20122 Milan, Italy

Department of Biomedical Sciences for Health, Università degli Studi di Milano, via Mangiagalli 31, 20133 Milan, Italy

IRCCS Fondazione Don Carlo Gnocchi ONLUS, via Capecelatro 66, 20148 Milan, Italy

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U.O.C. 1° Clinica Ortopedica, ASST Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Piazza Cardinal Ferrari 1, 20122 Milan, Italy

Author to whom correspondence should be addressed.

Cells 2020, 9(12), 2641; https://doi.org/10.3390/cells9122641

Received: 26 October 2020 / Revised: 4 December 2020 / Accepted: 7 December 2020 / Published: 8 December 2020

(This article belongs to the Section Cell Motility and Adhesion)

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Abstract

Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies. View Full-Text

Keywords: tendon; tenocytes; tendinopathy; collagen turnover; mechanotransduction; actin cytoskeleton; YAP/TAZ; medical device

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MDPI and ACS Style

Randelli, F.; Sartori, P.; Carlomagno, C.; Bedoni, M.; Menon, A.; Vezzoli, E.; Sommariva, M.; Gagliano, N. The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes. Cells 2020, 9, 2641. https://doi.org/10.3390/cells9122641

AMA Style

Randelli F, Sartori P, Carlomagno C, Bedoni M, Menon A, Vezzoli E, Sommariva M, Gagliano N. The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes. Cells. 2020; 9(12):2641. https://doi.org/10.3390/cells9122641

Chicago/Turabian Style

Randelli, Filippo, Patrizia Sartori, Cristiano Carlomagno, Marzia Bedoni, Alessandra Menon, Elena Vezzoli, Michele Sommariva, and Nicoletta Gagliano. 2020. "The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes" Cells 9, no. 12: 2641. https://doi.org/10.3390/cells9122641

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