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Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research

1
Banner Sun Health Research Institute, Sun City, AZ 85351, USA
2
School of Biological and Health Systems Engineering, Arizona State University, 501 E. Tyler Mall, ECG 334A, Tempe, AZ 85287, USA
3
Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85721, USA
4
Banner Alzheimer’s Institute, Phoenix, AZ 85006, USA
*
Author to whom correspondence should be addressed.
Cells 2020, 9(11), 2383; https://doi.org/10.3390/cells9112383
Received: 6 October 2020 / Accepted: 28 October 2020 / Published: 30 October 2020
The Arizona Study of Aging and Neurodegenerative Disorders/Brain and Body Donation Program at Banner Sun Health Research Institute (BSHRI) is a longitudinal clinicopathological study with a current enrollment of more than 900 living subjects for aging and neurodegenerative disease research. Annual clinical assessments are done by cognitive and movement neurologists and neuropsychologists. Brain and body tissues are collected at a median postmortem interval of 3.0 h for neuropathological diagnosis and banking. Since 2018, the program has undertaken banking of scalp fibroblasts derived from neuropathologically characterized donors with Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative diseases. Here, we describe the procedure development and cell characteristics from 14 male and 15 female donors (mean ± SD of age: 83.6 ± 12.2). Fibroblasts from explant cultures were banked at passage 3. The results of mRNA analysis showed positive expression of fibroblast activation protein, vimentin, fibronectin, and THY1 cell surface antigen. We also demonstrated that the banked fibroblasts from a postmortem elderly donor were successfully reprogramed to human-induced pluripotent stem cells (hiPSCs). Taken together, we have demonstrated the successful establishment of a human autopsy-derived fibroblast banking program. The cryogenically preserved cells are available for request at the program website of the BSHRI. View Full-Text
Keywords: Alzheimer’s disease; Parkinson’s disease; postmortem; scalp explant; fibroblasts; apolipoprotein E genotype; human inducible pluripotent stem cells; C9orf72 Alzheimer’s disease; Parkinson’s disease; postmortem; scalp explant; fibroblasts; apolipoprotein E genotype; human inducible pluripotent stem cells; C9orf72
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MDPI and ACS Style

Beh, S.T.; Frisch, C.; Brafman, D.A.; Churko, J.; Walker, J.E.; Serrano, G.E.; Sue, L.I.; Reiman, E.M.; Beach, T.G.; Lue, L.-F. Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research. Cells 2020, 9, 2383. https://doi.org/10.3390/cells9112383

AMA Style

Beh ST, Frisch C, Brafman DA, Churko J, Walker JE, Serrano GE, Sue LI, Reiman EM, Beach TG, Lue L-F. Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research. Cells. 2020; 9(11):2383. https://doi.org/10.3390/cells9112383

Chicago/Turabian Style

Beh, Suet T., Carlye Frisch, David A. Brafman, Jared Churko, Jessica E. Walker, Geidy E. Serrano, Lucia I. Sue, Eric M. Reiman, Thomas G. Beach, and Lih-Fen Lue. 2020. "Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research" Cells 9, no. 11: 2383. https://doi.org/10.3390/cells9112383

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