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Correction published on 30 October 2020, see Cells 2020, 9(11), 2380.
Open AccessArticle

Spontaneous Ultraslow Na+ Fluctuations in the Neonatal Mouse Brain

1
Institute of Neurobiology, Faculty of Mathematics and Natural Sciences, Heinrich Heine University Duesseldorf, 40225 Duesseldorf, Germany
2
Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, D-53105 Bonn, Germany
*
Author to whom correspondence should be addressed.
Cells 2020, 9(1), 102; https://doi.org/10.3390/cells9010102
Received: 25 November 2019 / Revised: 18 December 2019 / Accepted: 24 December 2019 / Published: 31 December 2019
(This article belongs to the Special Issue Astrocytes in Space and Time)
In the neonate forebrain, network formation is driven by the spontaneous synchronized activity of pyramidal cells and interneurons, consisting of bursts of electrical activity and intracellular Ca2+ oscillations. By employing ratiometric Na+ imaging in tissue slices obtained from animals at postnatal day 2–4 (P2–4), we found that 22% of pyramidal neurons and 43% of astrocytes in neonatal mouse hippocampus also exhibit transient fluctuations in intracellular Na+. These occurred at very low frequencies (~2/h), were exceptionally long (~8 min), and strongly declined after the first postnatal week. Similar Na+ fluctuations were also observed in the neonate neocortex. In the hippocampus, Na+ elevations in both cell types were diminished when blocking action potential generation with tetrodotoxin. Neuronal Na+ fluctuations were significantly reduced by bicuculline, suggesting the involvement of GABAA-receptors in their generation. Astrocytic signals, by contrast, were neither blocked by inhibition of receptors and/or transporters for different transmitters including GABA and glutamate, nor of various Na+-dependent transporters or Na+-permeable channels. In summary, our results demonstrate for the first time that neonatal astrocytes and neurons display spontaneous ultraslow Na+ fluctuations. While neuronal Na+ signals apparently largely rely on suprathreshold GABAergic excitation, astrocytic Na+ signals, albeit being dependent on neuronal action potentials, appear to have a separate trigger and mechanism, the source of which remains unclear at present. View Full-Text
Keywords: astrocytes; postnatal development; hippocampus; GABA; neuron-glia interaction astrocytes; postnatal development; hippocampus; GABA; neuron-glia interaction
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MDPI and ACS Style

Felix, L.; Ziemens, D.; Seifert, G.; Rose, C.R. Spontaneous Ultraslow Na+ Fluctuations in the Neonatal Mouse Brain. Cells 2020, 9, 102. https://doi.org/10.3390/cells9010102

AMA Style

Felix L, Ziemens D, Seifert G, Rose CR. Spontaneous Ultraslow Na+ Fluctuations in the Neonatal Mouse Brain. Cells. 2020; 9(1):102. https://doi.org/10.3390/cells9010102

Chicago/Turabian Style

Felix, Lisa; Ziemens, Daniel; Seifert, Gerald; Rose, Christine R. 2020. "Spontaneous Ultraslow Na+ Fluctuations in the Neonatal Mouse Brain" Cells 9, no. 1: 102. https://doi.org/10.3390/cells9010102

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