1. Introduction
2. Materials and Methods
2.1. FOXO1-DBD Expression and Purification
2.2. NMR Spectroscopy
2.3. NMR Structure Calculation
3. Results and Discussion
3.1. Solution Structure of the DNA-binding Domain of FOXO1
3.2. Comparison of FOXO1-DBD with Forkhead Domains of Other FOXO Proteins
3.3. Structural Differences Between Apo and DNA-bound Forms of FOXO1-DBD
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Non-Redundant Distance and Angle Constrains | |
---|---|
Total number of NOE constraints | 1712 |
Short-range NOEs | |
Intra-residue (i = j) | 445 |
Sequential (|i − j| = 1) | 402 |
Medium-range NOEs (1 < | i − j | < 5) | 325 |
Long-range NOEs (|i − j| ≥ 5) | 530 |
Torsion angles | 128 |
Hydrogen bond restrains | - |
Total number of restricting constraints | 1840 |
Total restricting constraints per restrained residue | 16.3 |
Residual constraint violations | |
Distance violations per structure | |
0.1–0.2 Å | 7.44 |
0.2–0.5 Å | 2.46 |
> 0.5 Å | 0 |
Root mean square (r.m.s.) of distance violation per constraint | 0.02 Å |
Maximum distance violation | 0.48 Å |
Dihedral angle viol. per structure | |
1–10° | 3.12 |
> 10° | 0 |
r.m.s. of dihedral violations per constraint | 0.55° |
Maximum dihedral angle viol. | 5.0° |
Ramachandran plot summary | |
Most favored regions | 98.3% |
Additionally allowed regions | 1.7% |
Generously allowed regions | 0.0% |
Disallowed regions | 0.0% |
r.m.s.d. to the mean structure | all/ordered 1 |
All backbone atoms | 10.0/0.4 Å |
All heavy atoms | 10.1/0.8 Å |
PDB entry | 6QVW |
BMRB accession code | 34364 |
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