Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Isolation and Culture
2.3. Gas Chromatography/Mass Spectrometry (GC/MS) Analyses
2.4. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Phospholipids
2.5. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Arachidonic Acid
2.6. LD Analysis by Confocal Microscopy
2.7. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AA | arachidonic acid |
ATGL | triacylglycerol lipase |
CE | cholesteryl esters |
DAG | diacylglycerol |
GC/MS | gas chromatography coupled to mass spectrometry |
LC/MS | liquid chromatography coupled to mass spectrometry |
LD | lipid droplet |
MAG | monoacylglycerol |
cPLA2α | group IVA cytosolic phospholipase A2α |
PL | phospholipids |
PC | choline-containing phospholipids |
PE | ethanolamine-containing phospholipids |
PI | phosphatidylinositol |
PS | phosphatidylserine |
TAG | triacylglycerol |
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Eicosanoid | LC Retention time (min) | [M−H]− (m/z) a | Product Ion (m/z) b |
---|---|---|---|
15-oxoETE | 14.30 | 317 | 219 |
12-oxoETE | 14.91 | 317 | 153 |
5-oxoETE | 15.78 | 317 | 203 |
15-HETE | 13.92 | 319 | 175, 219 |
11-HETE | 14.56 | 319 | 167 |
12-HETE | 14.93 | 319 | 179, 208 |
8-HETE | 15.01 | 319 | 155, 163 |
9-HETE | 15.15 | 319 | 151 |
5-HETE | 15.71 | 319 | 115 |
14,15-EET | 15.75 | 319 | 219 |
11,12-EET | 16.28 | 319 | 167, 179 |
8,9-EET | 16.31 | 319 | 155, 167 |
5,6-EET | 16.49 | 319 | 191 |
15-HPETE | 14.30 | 335 | 113 |
12-HPETE | 15.35 | 335 | 153 |
5-HPETE | 16.53 | 335 | 155 |
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Guijas, C.; Bermúdez, M.A.; Meana, C.; Astudillo, A.M.; Pereira, L.; Fernández-Caballero, L.; Balboa, M.A.; Balsinde, J. Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes. Cells 2019, 8, 941. https://doi.org/10.3390/cells8080941
Guijas C, Bermúdez MA, Meana C, Astudillo AM, Pereira L, Fernández-Caballero L, Balboa MA, Balsinde J. Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes. Cells. 2019; 8(8):941. https://doi.org/10.3390/cells8080941
Chicago/Turabian StyleGuijas, Carlos, Miguel A. Bermúdez, Clara Meana, Alma M. Astudillo, Laura Pereira, Lidia Fernández-Caballero, María A. Balboa, and Jesús Balsinde. 2019. "Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes" Cells 8, no. 8: 941. https://doi.org/10.3390/cells8080941