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Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface

1
Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
2
The Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA
3
Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA
4
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 813; https://doi.org/10.3390/cells8080813
Received: 3 July 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 2 August 2019
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Abstract

It has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature at the cellular and multicellular levels. To characterize and determine the mechanisms of curvature-dependent cell behaviors, we grow cells on open channels of the 60-µm radius. We found that cortical F-actin is 1.2-fold more enriched in epithelial cells grown on the curved surface compared to the flat control. We observed that myosin activity is required to promote cortical F-actin formation. Furthermore, cell–cell contact was shown to be indispensable for curvature-dependent cortical actin assembly. Our results indicate that the actomyosin network coupled with adherens junctions is involved in curvature-sensing at the multi-cellular level. View Full-Text
Keywords: curvature sensing; adherens junction; E-cadherin; cortical actin; myosin curvature sensing; adherens junction; E-cadherin; cortical actin; myosin
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Jung, W.-H.; Elawad, K.; Kang, S.H.; Chen, Y. Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface. Cells 2019, 8, 813.

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