Next Article in Journal
Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients with Advanced Epithelial Ovarian Cancer
Previous Article in Journal
Ciliary Proteins: Filling the Gaps. Recent Advances in Deciphering the Protein Composition of Motile Ciliary Complexes
Open AccessArticle

HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways

1
Department of Buco-Maxillofacial Surgery and Traumatology and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café-Subsetor Oeste-11 (N-11), 14040-904 Ribeirão Preto, SP, Brazil
2
Cell Culture Laboratory, School of Dentistry, Federal University of Pará (UFPA), Cidade Universitária Prof. José da Silveira Neto-R. Augusto Corrêa, 1-Guamá, 66075-110 Belém, PA, Brazil
3
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, R. Prof. Moacir Gomes de Freitas, 688-Pampulha, 31270-901 Belo Horizonte, MG, Brazil
4
Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374-Butantã, 05508-900 São Paulo, SP, Brazil
5
Department of Microbial Diseases, Eastman Dental Institute, University College London, 256 Grays Inn Rd, London WC1X 8LD, UK
*
Author to whom correspondence should be addressed.
Cells 2019, 8(7), 731; https://doi.org/10.3390/cells8070731
Received: 13 January 2019 / Revised: 8 July 2019 / Accepted: 15 July 2019 / Published: 17 July 2019
Background: The odontogenic keratocyst (OKC) is an odontogenic cyst that shows aggressive and intriguing biological behavior. It is suggested that a hypoxic environment occurs in OKC, which led us to investigate the immunoexpression and location of hypoxia-inducible factor 1-alpha (HIF-1α) and other hypoxia-related proteins. Methods: Twenty cases of OKC were evaluated for the expression of Notch homolog 1 (NOTCH1), HIF-1α, disintegrin and metalloproteinase domain-containing protein 12 (ADAM-12), and heparin-binding epidermal growth factor-like growth factor (HBEGF) by immunohistochemistry and compared to eight control cases of calcifying odontogenic cystic (COC), orthokeratinized odontogenic cyst (OOC), and normal oral mucosa (OM) in basal and parabasal layers. Results: In OKC, all the proteins tested were expressed significantly higher in both basal (except for NOTCH1 and HBEGF in OOC) and suprabasal epithelial layers compared to controls. Looking at the epithelial layers within OKC, we observed an increased NOTCH1 and HIF-1α expression in parabasal layers. Conclusions: These results suggest that hypoxia occurs more intensively in OKC compared to COC, OM, and OOC. Hypoxia appeared to be stronger in parabasal layers as observed by higher HIF-1α expression in upper cells. Overexpression of NOTCH1, ADAM-12, and HBEGF in OKC was observed, which suggests that microenvironmental hypoxia could potentially regulate the expression of hypoxia-related proteins, and consequently, its clinical and biological behavior. View Full-Text
Keywords: odontogenic keratocyst; HIF-1α; hypoxia; oxygen odontogenic keratocyst; HIF-1α; hypoxia; oxygen
Show Figures

Figure 1

MDPI and ACS Style

Miranda da Costa, N.M.; Saab Abe, C.T.; Mitre, G.P.; Mesquita, R.A.; Silva Kataoka, M.S.d.; Ribeiro Ribeiro, A.L.; Jaeger, R.G.; Alves-Júnior, S.d.M.; Smith, A.M.; Pinheiro, J.d.J.V. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways. Cells 2019, 8, 731. https://doi.org/10.3390/cells8070731

AMA Style

Miranda da Costa NM, Saab Abe CT, Mitre GP, Mesquita RA, Silva Kataoka MSd, Ribeiro Ribeiro AL, Jaeger RG, Alves-Júnior SdM, Smith AM, Pinheiro JdJV. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways. Cells. 2019; 8(7):731. https://doi.org/10.3390/cells8070731

Chicago/Turabian Style

Miranda da Costa, Natacha M.; Saab Abe, Caio T.; Mitre, Geovanni P.; Mesquita, Ricardo A.; Silva Kataoka, Maria S.d.; Ribeiro Ribeiro, André L.; Jaeger, Ruy G.; Alves-Júnior, Sérgio d.M.; Smith, Andrew M.; Pinheiro, João d.J.V. 2019. "HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways" Cells 8, no. 7: 731. https://doi.org/10.3390/cells8070731

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop