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Cells 2018, 7(7), 80; https://doi.org/10.3390/cells7070080

Sub-Cellular Localization of Metalloproteinases in Megakaryocytes

1
Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy
2
Laboratory of Biotechnology, IRCCS San Matteo Foundation, 27100 Pavia, Italy
3
Section of Clinical Biochemistry and Molecular Genetics, Department of Biomolecular Sciences, University “Carlo Bo” of Urbino, 61029 Urbino, Italy
4
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
These authors equally contributed to this work.
These authors are both co-senior authors.
*
Author to whom correspondence should be addressed.
Received: 13 June 2018 / Revised: 17 July 2018 / Accepted: 18 July 2018 / Published: 20 July 2018
(This article belongs to the Special Issue Extracellular Matrix Remodeling)
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Abstract

Metalloproteinases (MMPs) are zinc-dependent endopeptidases that play essential roles as the mediator of matrix degradation and remodeling during organogenesis, wound healing and angiogenesis. Although MMPs were originally identified as matrixin proteases that act in the extracellular matrix, more recent research has identified members of the MMP family in unusual locations within the cells, exerting distinct functions in addition to their established role as extracellular proteases. During thrombopoiesis, megakaryocytes (Mks) sort MMPs to nascent platelets through pseudopodial-like structure known as proplatelets. Previous studies identified gelatinases, MMP-2 and MMP-9, as a novel regulator system of Mks and the platelet function. In this work we have exploited a sensitive immunoassay to detect and quantify multiple MMP proteins and their localization, in conditioned medium and sub-cellular fractions of primary human CD34+-derived Mks. We provide evidence that Mks express other MMPs in addition to gelatinases MMP-2 and MMP-9, peculiar isoforms of MMP-9 and MMPs with a novel nuclear compartmentalization. View Full-Text
Keywords: megakaryocyte; metalloproteinase; thrombopoiesis megakaryocyte; metalloproteinase; thrombopoiesis
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Malara, A.; Ligi, D.; Di Buduo, C.A.; Mannello, F.; Balduini, A. Sub-Cellular Localization of Metalloproteinases in Megakaryocytes. Cells 2018, 7, 80.

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