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Cells 2014, 3(4), 1131-1158;

A Phospho-SIM in the Antiviral Protein PML is Required for Its Recruitment to HSV-1 Genomes

Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA
Stowers Institute for Medical Research Flow Cytometry Facility, Kansas City, MO 64110, USA
Mass Spectrometry and Proteomics Resource Laboratory, Harvard University, Cambridge, MA 02138, USA
Current address: Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655, USA
Author to whom correspondence should be addressed.
Received: 21 July 2014 / Revised: 8 October 2014 / Accepted: 3 November 2014 / Published: 10 December 2014
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Herpes simplex virus type 1 (HSV-1) is a significant human pathogen that infects a large portion of the human population. Cells deploy a variety of defenses to limit the extent to which the virus can replicate. One such factor is the promyelocytic leukemia (PML) protein, the nucleating and organizing factor of nuclear domain 10 (ND10). PML responds to a number of stimuli and is implicated in intrinsic and innate cellular antiviral defenses against HSV-1. While the role of PML in a number of cellular pathways is controlled by post-translational modifications, the effects of phosphorylation on its antiviral activity toward HSV-1 have been largely unexplored. Consequently, we mapped phosphorylation sites on PML, mutated these and other known phosphorylation sites on PML isoform I (PML-I), and examined their effects on a number of PML’s activities. Our results show that phosphorylation at most sites on PML-I is dispensable for the formation of ND10s and colocalization between PML-I and the HSV-1 regulatory protein, ICP0, which antagonizes PML-I function. However, inhibiting phosphorylation at sites near the SUMO-interaction motif (SIM) of PML-I impairs its ability to respond to HSV-1 infection. Overall, our data suggest that PML phosphorylation regulates its antiviral activity against HSV-1. View Full-Text
Keywords: PML; ND10; PML-NB; phosphorylation; HSV; ICP0; intrinsic immunity PML; ND10; PML-NB; phosphorylation; HSV; ICP0; intrinsic immunity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Smith, M.C.; Box, A.C.; Haug, J.S.; Lane, W.S.; Davido, D.J. A Phospho-SIM in the Antiviral Protein PML is Required for Its Recruitment to HSV-1 Genomes. Cells 2014, 3, 1131-1158.

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