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Volume 14
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10.3390/cells14211736
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Article

The BUD31 Homologous Gene in Schizosaccharomyces pombe Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway

by
Ildikó Vig
1,2,
Lajos Acs-Szabo
1,3,
Zsigmond Benkő
2,4,
Silvia Bagelova Polakova
4,5,
László Attila Papp
1,
Juraj Gregan
4,6 and
Ida Miklós
1,*
1
Department of Genetics and Applied Microbiology, Faculty of Science and Technology, Institute of Biotechnology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary
2
Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, Institute of Biotechnology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary
3
Department of Botany, Faculty of Science and Technology, Institute of Biology and Ecology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary
4
Department of Chromosome Biology, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr-Gasse 9, 1030 Vienna, Austria
5
Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, 840 05 Bratislava, Slovakia
6
Institute of Microbial Genetics, Department of Agricultural Sciences, BOKU University Vienna, Campus Tulln, Konrad Lorenz Strasse 24, 3430 Tulln an der Donau, Austria
*
Author to whom correspondence should be addressed.
Cells 2025, 14(21), 1736; https://doi.org/10.3390/cells14211736
Submission received: 25 September 2025 / Revised: 30 October 2025 / Accepted: 31 October 2025 / Published: 5 November 2025
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Abstract

The human BUD31 gene has been associated with various processes including cancer. To better understand its function, we used genetic methods to study Schizosaccharomyces pombe cells lacking the BUD31 homologous gene (cwf14) and performed sequence analysis using bioinformatics methods. Mutant cells lacking the cwf14 gene showed cell size and division defects, altered stress response, rapamycin sensitivity, enhanced chronological aging, and increased sporulation tendency. These processes are known to be regulated by the TOR pathway. The cwf14-TOR link was also supported by further experiments. We demonstrated that most protein-coding genes affected by cwf14 deletion are upregulated, encode hydrolases, oxidoreductases, and are often involved in transport. GO enrichment drew our attention to genes related to nitrogen transport, while additional data pointed to a nutrient/nitrogen (N) sensing problem. Although Cwf14 protein is associated with spliceosome complex, most genes affected by the absence of cwf14 do not contain introns, suggesting that they are influenced indirectly by the cwf14 gene. In silico experiments have revealed that BUD31 orthologous genes are found from yeast to humans, are evolutionarily conserved with a high degree of sequence identity, conserved motifs, and structures. Since the human gene partially complemented the mutant phenotype of S. pombe cells, indicating functional homology, our data can help better understand pathological mechanisms observed in human cancer cells.
Keywords: BUD31 gene; splicing; TOR-pathway; Schizosaccharomyces pombe; interspecific complementation; cell division BUD31 gene; splicing; TOR-pathway; Schizosaccharomyces pombe; interspecific complementation; cell division

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MDPI and ACS Style

Vig, I.; Acs-Szabo, L.; Benkő, Z.; Bagelova Polakova, S.; Papp, L.A.; Gregan, J.; Miklós, I. The BUD31 Homologous Gene in Schizosaccharomyces pombe Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway. Cells 2025, 14, 1736. https://doi.org/10.3390/cells14211736

AMA Style

Vig I, Acs-Szabo L, Benkő Z, Bagelova Polakova S, Papp LA, Gregan J, Miklós I. The BUD31 Homologous Gene in Schizosaccharomyces pombe Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway. Cells. 2025; 14(21):1736. https://doi.org/10.3390/cells14211736

Chicago/Turabian Style

Vig, Ildikó, Lajos Acs-Szabo, Zsigmond Benkő, Silvia Bagelova Polakova, László Attila Papp, Juraj Gregan, and Ida Miklós. 2025. "The BUD31 Homologous Gene in Schizosaccharomyces pombe Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway" Cells 14, no. 21: 1736. https://doi.org/10.3390/cells14211736

APA Style

Vig, I., Acs-Szabo, L., Benkő, Z., Bagelova Polakova, S., Papp, L. A., Gregan, J., & Miklós, I. (2025). The BUD31 Homologous Gene in Schizosaccharomyces pombe Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway. Cells, 14(21), 1736. https://doi.org/10.3390/cells14211736

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