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Cells
Volume 14
Issue 11
10.3390/cells14110799
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model

by
María Del Mar Figueroa-Gispert
1,
Claudia M. Ramos-Lugo
1,
Carlimar Ocasio-Malavé
1,
Rizaldy P. Scott
2,
Jared T. Ahrendsen
2,
Mercedes Gomez-Samblas
3,
Antonio Osuna
3,
Stephanie M. Dorta-Estremera
1 and
Ana M. Espino
1,*
1
Department of Microbiology and Medical Zoology, University of Puerto Rico-Medical Sciences Campus, San Juan, PR 00936, USA
2
Mouse Histology & Phenotyping Laboratory, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
3
Institute of Biotechnology, Department of Parasitology, University of Granada, 18071 Granada, Spain
*
Author to whom correspondence should be addressed.
Cells 2025, 14(11), 799; https://doi.org/10.3390/cells14110799
Submission received: 1 May 2025 / Revised: 15 May 2025 / Accepted: 20 May 2025 / Published: 29 May 2025
Review Reports Versions Notes

Abstract

Ulcerative colitis (UC) is the most prevalent inflammatory bowel disease (IBD) in the USA. Current treatments present clinical limitations, underscoring the need for innovative therapeutics that promote an anti-inflammatory immune response. This study evaluates the anti-inflammatory potential of Fh15, a recombinant Fasciola hepatica fatty acid binding protein, in a DSS-induced UC mouse model. Our results demonstrated that Fh15 treatment significantly ameliorated the severity of colitis by reducing the disease activity index (DAI) and histopathological scores. Moreover, Fh15 also decreased the serum levels of myeloperoxidase (MPO) and chitinase-3-like protein 1 (CHI3L1), and the expression of S100A9, a calcium and zinc binding protein, which is an important marker for the pathogenesis of UC. Furthermore, Fh15 downregulated pro-inflammatory cytokines TNFα and IL-1β in the distal colon, suggesting modulation of macrophage activity. Immunohistochemistry analysis revealed significantly reduced neutrophil and macrophage infiltration in UC Fh15-treated mice. These findings highlight the therapeutic potential of Fh15 for UC, as it modulates inflammatory responses, reduces leukocyte infiltration, and preserves colon integrity.
Keywords: ulcerative colitis; Fasciola hepatica; fatty acid binding protein; myeloperoxidase; chitinase-3 like-protein-1; S100A9; TNF-α; IL-1β; neutrophils; macrophages ulcerative colitis; Fasciola hepatica; fatty acid binding protein; myeloperoxidase; chitinase-3 like-protein-1; S100A9; TNF-α; IL-1β; neutrophils; macrophages

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MDPI and ACS Style

Figueroa-Gispert, M.D.M.; Ramos-Lugo, C.M.; Ocasio-Malavé, C.; Scott, R.P.; Ahrendsen, J.T.; Gomez-Samblas, M.; Osuna, A.; Dorta-Estremera, S.M.; Espino, A.M. Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model. Cells 2025, 14, 799. https://doi.org/10.3390/cells14110799

AMA Style

Figueroa-Gispert MDM, Ramos-Lugo CM, Ocasio-Malavé C, Scott RP, Ahrendsen JT, Gomez-Samblas M, Osuna A, Dorta-Estremera SM, Espino AM. Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model. Cells. 2025; 14(11):799. https://doi.org/10.3390/cells14110799

Chicago/Turabian Style

Figueroa-Gispert, María Del Mar, Claudia M. Ramos-Lugo, Carlimar Ocasio-Malavé, Rizaldy P. Scott, Jared T. Ahrendsen, Mercedes Gomez-Samblas, Antonio Osuna, Stephanie M. Dorta-Estremera, and Ana M. Espino. 2025. "Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model" Cells 14, no. 11: 799. https://doi.org/10.3390/cells14110799

APA Style

Figueroa-Gispert, M. D. M., Ramos-Lugo, C. M., Ocasio-Malavé, C., Scott, R. P., Ahrendsen, J. T., Gomez-Samblas, M., Osuna, A., Dorta-Estremera, S. M., & Espino, A. M. (2025). Fh15 Reduces Colonic Inflammation and Leukocyte Infiltration in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model. Cells, 14(11), 799. https://doi.org/10.3390/cells14110799

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