JAK/STAT Inhibition Normalizes Lipid Composition in 3D Human Epidermal Equivalents Challenged with Th2 Cytokines
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Chemicals
2.3. Culture of 3D Epidermal Equivalents and Stimulation with Cytokines and a JAK1/3 Inhibitor
2.4. Isolation of RNA and Analysis of mRNA by Real-Time RT-PCR
2.5. Western Blot Analysis
2.6. Histology, Morphometry, and Immunofluorescence Analysis of HEEs
2.7. Lipid Extraction
2.8. GCMS Analysis
2.9. LC Separations
2.10. HRMS
2.11. Data Processing and Statistical Analysis
3. Results
3.1. Effects of JAK/STAT Inhibition by Tofacitinib on Th2 Cytokine-Mediated Changes in Epidermal Morphology and Barrier Protein Expression
3.2. JAK/STAT Inhibition Counteracts the Induction of Inflammatory Genes in Th2-Treated Epidermal Equivalents
3.3. Modulation of Lipid Genes by Th2 Cytokines and Counteracting Effects of Tofacitinib
3.4. Alteration of Lipid Profiles Induced by Th2 Cytokines and Modulating Effects of Tofacitinib
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Flori, E.; Cavallo, A.; Mosca, S.; Kovacs, D.; Cota, C.; Zaccarini, M.; Di Nardo, A.; Bottillo, G.; Maiellaro, M.; Camera, E.; et al. JAK/STAT Inhibition Normalizes Lipid Composition in 3D Human Epidermal Equivalents Challenged with Th2 Cytokines. Cells 2024, 13, 760. https://doi.org/10.3390/cells13090760
Flori E, Cavallo A, Mosca S, Kovacs D, Cota C, Zaccarini M, Di Nardo A, Bottillo G, Maiellaro M, Camera E, et al. JAK/STAT Inhibition Normalizes Lipid Composition in 3D Human Epidermal Equivalents Challenged with Th2 Cytokines. Cells. 2024; 13(9):760. https://doi.org/10.3390/cells13090760
Chicago/Turabian StyleFlori, Enrica, Alessia Cavallo, Sarah Mosca, Daniela Kovacs, Carlo Cota, Marco Zaccarini, Anna Di Nardo, Grazia Bottillo, Miriam Maiellaro, Emanuela Camera, and et al. 2024. "JAK/STAT Inhibition Normalizes Lipid Composition in 3D Human Epidermal Equivalents Challenged with Th2 Cytokines" Cells 13, no. 9: 760. https://doi.org/10.3390/cells13090760