Aptamer-Hytac Chimeras for Targeted Degradation of SARS-CoV-2 Spike-1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Oligonucleotides and Aptamers Preparation
2.2. Conjugation of Aptamers with Hytacs
2.2.1. Conjugation of Aptamers with Adamantyl Groups
2.2.2. Conjugation of Aptamers with Boc2-Arg and Boc3-Arg
2.3. Generation of Vectors Expressing S1
2.4. Cell Transfection
2.5. Detection of Aptamers and Aptamers-Hytacs
2.6. Generation of Cells Lines Expressing Stable S1
2.7. Flow Cytometry
2.8. Immunoblotting Analysis
2.9. Degradation Assays Using Aptamer-Hytacs
3. Results
3.1. Conjugation of Aptamers to Hytacs
3.2. Cell Lines Stably Expressing S1
3.3. Efficiency of Aptamer Delivery to Human Cells
3.4. Effect of Hytac-Free Aptamers on S1 Levels
3.5. Degradation of Spike S1 by Aptamers-Hytacs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fàbrega, C.; Gallisà-Suñé, N.; Zuin, A.; Ruíz, J.S.; Coll-Martínez, B.; Fabriàs, G.; Eritja, R.; Crosas, B. Aptamer-Hytac Chimeras for Targeted Degradation of SARS-CoV-2 Spike-1. Cells 2024, 13, 1767. https://doi.org/10.3390/cells13211767
Fàbrega C, Gallisà-Suñé N, Zuin A, Ruíz JS, Coll-Martínez B, Fabriàs G, Eritja R, Crosas B. Aptamer-Hytac Chimeras for Targeted Degradation of SARS-CoV-2 Spike-1. Cells. 2024; 13(21):1767. https://doi.org/10.3390/cells13211767
Chicago/Turabian StyleFàbrega, Carme, Núria Gallisà-Suñé, Alice Zuin, Juan Sebastián Ruíz, Bernat Coll-Martínez, Gemma Fabriàs, Ramon Eritja, and Bernat Crosas. 2024. "Aptamer-Hytac Chimeras for Targeted Degradation of SARS-CoV-2 Spike-1" Cells 13, no. 21: 1767. https://doi.org/10.3390/cells13211767