Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Irradiation
2.2. Behavioural Studies
2.2.1. Open Field Test
2.2.2. Light Dark Box
2.2.3. Elevated Plus Maze
2.2.4. Tail Suspension Test
2.2.5. Forced Swim Test
2.3. Sample Collection
2.4. Immunohistochemical Staining
2.5. RNA Extraction from the Hippocampus and Whole Blood
2.6. Systematic miRNA Sequencing (miRSeq) and mRNA Sequencing Analysis
2.7. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) Analysis of mRNA
2.8. Real-Time RT-PCR Analysis for miRNA
2.9. Statistical Analyses
3. Results
3.1. Body Weight and Behavioural Changes
3.2. Immunohistochemistry Examination
3.3. mRNA Sequencing and qRT-PCR in the Hippocampus
3.4. miRNA Sequencing and qRT-PCR in the Hippocampus
3.5. mRNA Sequencing and qRT-PCR in the Blood
3.6. miRNA Sequencing and qRT-PCR in the Blood
3.7. Venn Diagram Analysis of Differentially Expressed mRNAs and miRNAs between Blood and Hippocampus
4. Discussion
4.1. Main Findings
4.2. Chronic Low-Dose-Rate Irradiation Did Not Induce Abnormal Cellular Changes in the Dentate Gyrus of the Hippocampus and Neuropsychiatric Abnormality
4.3. Chronic Low-Dose-Rate Irradiation-Induced Persistent DNA Damage Foci
4.4. Chronic Low-Dose-Rate Irradiation Induced miRNA and mRNA Changes in the Hippocampus
4.5. Chronic Low-Dose-Rate Irradiation Induced miRNA and mRNA Changes in the Blood
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Primer Sequence |
---|---|
Ccn1 F | CGTCCTTGTGGACAACCAGT |
Ccn1 R | CATGATGCTTGCGCTTCTCC |
Cort F | TGTGAGATGCCAACGAGACC |
Cort R | TGTTGTCGGTAGCGAGCATT |
Foxh1 F | CAGGCTGAAACTGGCTCAGA |
Foxh1 R | AGGAGCTAGAGGGTCCAGTG |
Fli1 F | ATCTGAAGGGGCTACGAGGT |
Fli1 R | TGACTCTCCGTTCGTTGGTG |
Opalin | GATGAGCCCCGTATGTCCTG |
Opalin | GCCTGTCCTAACTTGTGCCA |
Fosb F | CCTTCAGTCCCAAAGACGAGT |
Fosb R | GGGTGGGGTTTGGGATTAGG |
Ets1 | CGGTCAGCGGGAATTTGAGA |
Ets1 | ATCTCCTGGCCACCTCATCT |
Hs3st5 | GGCGTGTCTGAATGTAGGCT |
Hs3st5 | CTCCTTCCCCTCTAGCACCT |
Eif4ebp2 F | AGCAGAAGTGCCAACACCTT |
Eif4ebp2 R | GATGTGGAAAATGGCCCGGT |
Zfhx4 F | TAAGGCTGAGACTTGGCTGC |
Zfhx4 R | CCCTGTCAGGCTCTATCCCT |
Yod1 F | TTTGACCCCATTTCCCCAGT |
Yod1 R | TAGGTTGGCCAGTAACCCCT |
Hivep3 F | CCCACCATCCCCACTGAAAG |
Hivep3 R | GGCAACCCGGGCTCCTTTAT |
Chrm5 F | GCTTGTCAAGGTGCAAGGTC |
Chrm5 R | GTCCCTGCTGTTCTTCACAGA |
Cox6b2 F | TTTTCTCCCGTGCTCTTGGG |
Cox6b2 R | AGTACTCGCAGGGTTGTGTG |
Tspo F | CTTGGGTCTCTACACTGGTC |
Tspo R | AGACTTTATTTAGCTTTAAAACACC |
Notch3 F | CTCTCCCTGCCTCAACTTCC |
Notch3 R | CTCCCAAATGTCCCCTGACC |
Kifc1 F | GAGCCTGCAAAGAAACGGAC |
Kifc1 R | TATATGCCACCTACTGCCAGG |
Orc1 F | AAGTGTTGGAGAAGTTACGGT |
Orc1 R | GACCAACCCACCAGGGATTT |
Apoc1 F | GGGCGGTGGTGAATACTAGC |
Apoc1 R | TGGCTACGACCACAATCAGG |
Rnf43 F | AATTTGTTTCATCCCCGTGCC |
Rnf43 R | CTCCCATCGTCACTGCGAAT |
Ahnak F | CAGTCAGCACTGCGACCTC |
Ahnak R | TTTGCAGGACTCTGCTCAGG |
Tppp3 F | TAGAAGCCGGGTGGCATGG |
Tppp3 R | GTTCTTTGTGGGAGCCCGTA |
Cdkn2c F | CCGGCACAGTACCTTCAGAG |
Cdkn2c R | AGCTCAGGCTCTTCACTGCAA |
S1pr5 F | ACACCAAATGCCCAGCTTAC |
S1pr5 R | AAGTCTCCTGTAACCGGCAC |
Mcpt4 F | AGAATCTCTCTCCAAGCTGT |
Mcpt4 R | GTAAGGGCGAGAATGTGGTC |
Rbpms F | ATTGCCTCAAGAGGAGCAGG |
Rbpms R | GGGCGGTCTATCTGACATGG |
Vcam1 F | ACTTTCTAATTCATGGTAGAATGGC |
Vcam1 R | CAATGAAGAAACAGGTCCCCG |
Lepr F | TGATAATGGTGTGACGGTTGC |
Lepr R | GGAAGCTTTCACACACTGAA |
Slc15a2 F | CAGGGAACGAGCTTGGGAAT |
Slc15a2 R | GCAGTTGTCTGGGGAAAGGA |
Mlxipl F | CCTGAGCATCTGCAGCCTC |
Mlxipl R | ATGACAGCCTCAGGTTTCCG |
Gapdh F | ACCACAGTCCATGCCATCAC |
Gapdh R | TCCACCACCCTGTTGCTGTA |
miRNA | Primer Sequence |
---|---|
mmu-miR-448-3p | TTGCATATGTAGGATGTCCCAT |
mmu-miR-193a-3p | AACTGGCCTACAAAGTCCCAGT |
mmu-miR-361-5p | TTATCAGAATCTCCAGGGGTAC |
mmu-miR-450-5p | CGTTTTGCGATGTGTTCCTAAT |
mmu-miR-20a-3p | ACTGCATTACGAGCACTTAAAG |
mmu-miR-495-3p | AAACAAACATGGTGCACTTCTT |
mmu-miR-199b-3p | ACAGTAGTCTGCACATTGGTTA |
mmu-miR-101a-5p | TCAGTTATCACAGTGCTGATGC |
mmu-miR-10b-5p | TACCCTGTAGAACCGAATTTGTG |
mmu-miR-1298-3p | CATCTGGGCAACTGATTGAACT |
mmu-miR-124b-3p | TCAAGGTCCGCTGTGAACACGG |
mmu-miR-16-2-3p | GACCAATATTATTGTGCTGCTTT |
mmu-miR-7037-5p | AAGGTGGCCACAGGAGATCATGGT |
mmu-miR-206-3p | TGGAATGTAAGGAAGTGTGTGG |
mmu-miR-1947-5p | AGGACGAGCTAGCTGAGTGCTG |
mmu-miR-199b-3p | ACAGTAGTCTGCACATTGGTTA |
mmu-miR-5134-3p | ACGGGTGGCCCTCTTTCTGCAG |
mmu-miR-296-5p | AGGGCCCCCCCTCAATCCTGT |
mmu-miR-7024-5p | TTGGGGGATGGGTTGCTTGGC |
mmu-miR-6967-3p | TCATCTTTATCTCTCCCCAG |
mmu-miR-68 | GCTGTACTGACTTGATGAAAGTAC |
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Wang, H.; Lau, S.; Tan, A.; Tang, F.R. Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood. Cells 2024, 13, 1705. https://doi.org/10.3390/cells13201705
Wang H, Lau S, Tan A, Tang FR. Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood. Cells. 2024; 13(20):1705. https://doi.org/10.3390/cells13201705
Chicago/Turabian StyleWang, Hong, Salihah Lau, Amanda Tan, and Feng Ru Tang. 2024. "Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood" Cells 13, no. 20: 1705. https://doi.org/10.3390/cells13201705
APA StyleWang, H., Lau, S., Tan, A., & Tang, F. R. (2024). Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood. Cells, 13(20), 1705. https://doi.org/10.3390/cells13201705