3D-Bioprinted Co-Cultures of Glioblastoma Multiforme and Mesenchymal Stromal Cells Indicate a Role for Perivascular Niche Cells in Shaping Glioma Chemokine Microenvironment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bioinformatic Data Analysis
2.2. Glioblastoma Multiforme Cell Lines Culture
2.3. Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adipose Tissue
2.4. 3D Bioprinting
2.5. Bioprinting of 3D Glioblastoma Multiforme Constructs
2.6. Collection of Samples and Chemokine Profiling
2.7. Luminex
2.8. ELISA
2.9. Statistical Analysis
3. Results
3.1. Gene Expression Analysis from the TCGA and CCGA Cohorts Revealed a Broad Chemokine Expression Profile in GBM Tissues
3.2. Glioblastoma Multiforme Primary Cell Cultures Show a Markedly Reduced Chemokine Expression Profile Compared to the Tumor Tissues from Which They Originated
3.3. 3D Bioprinting of Tumor-Derived Primary GBM Cells with Cells of the Tumor Microenvironment Partially Restores the Diversity of Expressed Chemokines
3.4. Single Cell RNA-Seq Data Enabled Identification of the Cellular Source of Expressed Chemokines in GBM Tumors
3.5. The Use of 3D-Bioprinted Co-Cultures of GBM and Mesenchymal Cells Reveals the Profound Effect of Cell–Cell Interactions, Type of Biomaterial, and Oxygen Partial Pressure on Chemokine Secretion
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zielniok, K.; Rusinek, K.; Słysz, A.; Lachota, M.; Bączyńska, E.; Wiewiórska-Krata, N.; Szpakowska, A.; Ciepielak, M.; Foroncewicz, B.; Mucha, K.; et al. 3D-Bioprinted Co-Cultures of Glioblastoma Multiforme and Mesenchymal Stromal Cells Indicate a Role for Perivascular Niche Cells in Shaping Glioma Chemokine Microenvironment. Cells 2024, 13, 1404. https://doi.org/10.3390/cells13171404
Zielniok K, Rusinek K, Słysz A, Lachota M, Bączyńska E, Wiewiórska-Krata N, Szpakowska A, Ciepielak M, Foroncewicz B, Mucha K, et al. 3D-Bioprinted Co-Cultures of Glioblastoma Multiforme and Mesenchymal Stromal Cells Indicate a Role for Perivascular Niche Cells in Shaping Glioma Chemokine Microenvironment. Cells. 2024; 13(17):1404. https://doi.org/10.3390/cells13171404
Chicago/Turabian StyleZielniok, Katarzyna, Kinga Rusinek, Anna Słysz, Mieszko Lachota, Ewa Bączyńska, Natalia Wiewiórska-Krata, Anna Szpakowska, Martyna Ciepielak, Bartosz Foroncewicz, Krzysztof Mucha, and et al. 2024. "3D-Bioprinted Co-Cultures of Glioblastoma Multiforme and Mesenchymal Stromal Cells Indicate a Role for Perivascular Niche Cells in Shaping Glioma Chemokine Microenvironment" Cells 13, no. 17: 1404. https://doi.org/10.3390/cells13171404