Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D
Abstract
:1. Introduction
2. Materials and Methods
2.1. Maintenance of Immortalized Porcine Liver-Derived Endothelial Cells (ipLDEC)
2.2. CRISPR/Cas9-Mediated Disruption of the Porcine ULBP-1 Gene
2.3. Binding of pULBP-1 Deficient Cells to Human NKG2D-Fc Chimera Protein
2.4. Human NK Cell Activation in Response to Porcine Endothelial Cell Stimulation
2.5. Calcein-AM Release Assay
2.6. Statistical Analysis
3. Results
3.1. NKG2D Plays a Pivotal Role in Human NK Cell-to-Pig Endothelial Cell Xenogeneic Immune Response
3.2. Disruption of pULBP-1 Gene in 5GKO Cells
3.3. pULBP-1-Deficient Cells Exhibit a Reduced Binding Potential to NKG2D-Fc Chimera Protein
3.4. pULBP-1 Does Not Contribute to Xenogeneic Human NK Cell Activation
3.5. pULBP-1 on Porcine Cells Has No Effect on Human NK Cell-Mediated Cytotoxicity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lopez, K.J.; Spence, J.P.; Li, W.; Zhang, W.; Wei, B.; Cross-Najafi, A.A.; Butler, J.R.; Cooper, D.K.C.; Ekser, B.; Li, P. Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D. Cells 2023, 12, 2587. https://doi.org/10.3390/cells12222587
Lopez KJ, Spence JP, Li W, Zhang W, Wei B, Cross-Najafi AA, Butler JR, Cooper DKC, Ekser B, Li P. Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D. Cells. 2023; 12(22):2587. https://doi.org/10.3390/cells12222587
Chicago/Turabian StyleLopez, Kevin J., John Paul Spence, Wei Li, Wenjun Zhang, Barry Wei, Arthur A. Cross-Najafi, James R. Butler, David K. C. Cooper, Burcin Ekser, and Ping Li. 2023. "Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D" Cells 12, no. 22: 2587. https://doi.org/10.3390/cells12222587
APA StyleLopez, K. J., Spence, J. P., Li, W., Zhang, W., Wei, B., Cross-Najafi, A. A., Butler, J. R., Cooper, D. K. C., Ekser, B., & Li, P. (2023). Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D. Cells, 12(22), 2587. https://doi.org/10.3390/cells12222587