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Cells
Volume 11
Issue 9
10.3390/cells11091587
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Article

Surface Hydration Protects Cystic Fibrosis Airways from Infection by Restoring Junctional Networks

by
Juliette L. Simonin
1,
Alexandre Luscher
2,
Davide Losa
1,†,
Mehdi Badaoui
1,
Christian van Delden
2,3,
Thilo Köhler
2 and
Marc Chanson
1,*
1
Department of Cell Physiology & Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland
2
Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland
3
Department of Medicine Specialties, Division of Infectious Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
†
Present address: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, 38123 Trento, TN, Italy.
Academic Editors: Teresinha Leal, Tristan Montier, Martial Delion and Angélique Mottais
Cells 2022, 11(9), 1587; https://doi.org/10.3390/cells11091587
Received: 17 March 2022 / Revised: 20 April 2022 / Accepted: 6 May 2022 / Published: 9 May 2022
(This article belongs to the Collection Cystic Fibrosis: Cells, Physiopathology and Emerging Therapies)
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Abstract

Defective hydration of airway surface mucosa is associated with recurrent lung infection in cystic fibrosis (CF), a disease caused by CF transmembrane conductance regulator (CFTR) gene mutations. Whether the composition and/or presence of an airway surface liquid (ASL) is sufficient to prevent infection remains unclear. The susceptibility to infection of polarized wild type and CFTR knockdown (CFTR-KD) airway epithelial cells was determined in the presence or absence of a healthy ASL or physiological saline. CFTR-KD epithelia exhibited strong ASL volume reduction, enhanced susceptibility to infection, and reduced junctional integrity. Interestingly, the presence of an apical physiological saline alleviated disruption of the airway epithelial barrier by stimulating essential junctional protein expression. Thus, rehydrated CFTR-KD cells were protected from infection despite normally intense bacterial growth. This study indicates that an epithelial integrity gatekeeper is modulated by the presence of an apical liquid volume, irrespective of the liquid’s composition and of expression of a functional CFTR. View Full-Text
Keywords: mucosal immunity; P. aeruginosa; airway surface liquid; epithelium integrity; cystic fibrosis mucosal immunity; P. aeruginosa; airway surface liquid; epithelium integrity; cystic fibrosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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    Doi: 10.26037/yareta:ckvglc7mgbdrjml5k6nllgtlhe
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MDPI and ACS Style

Simonin, J.L.; Luscher, A.; Losa, D.; Badaoui, M.; van Delden, C.; Köhler, T.; Chanson, M. Surface Hydration Protects Cystic Fibrosis Airways from Infection by Restoring Junctional Networks. Cells 2022, 11, 1587. https://doi.org/10.3390/cells11091587

AMA Style

Simonin JL, Luscher A, Losa D, Badaoui M, van Delden C, Köhler T, Chanson M. Surface Hydration Protects Cystic Fibrosis Airways from Infection by Restoring Junctional Networks. Cells. 2022; 11(9):1587. https://doi.org/10.3390/cells11091587

Chicago/Turabian Style

Simonin, Juliette L., Alexandre Luscher, Davide Losa, Mehdi Badaoui, Christian van Delden, Thilo Köhler, and Marc Chanson. 2022. "Surface Hydration Protects Cystic Fibrosis Airways from Infection by Restoring Junctional Networks" Cells 11, no. 9: 1587. https://doi.org/10.3390/cells11091587

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