Multiomics Approach Reveals an Important Role of BNIP3 in Myocardial Remodeling and the Pathogenesis of Heart Failure with Reduced Ejection Fraction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production of Recombinant Adenoviruses and Adeno-Associated Virus
2.2. Isolation and Culture of Adult Rat Cardiomyocytes and Design of In Vitro Experiments
2.2.1. Assessment of Mt-Reactive Oxygen Species (ROS) and Mt-Membrane Potential
2.2.2. Measurement of Mt-Oxygen (O2) Flux (JO2) and Oxidation Phosphorylation (OXPHOS)
2.3. Animal Model of Ascending Aortic Banding and Gene Delivery
2.4. Discovery-Based Proteomic and Phosphoproteomic Quantitative Analysis Using Isobaric Labeling and Liquid Chromatography–Mass Spectrometry
2.5. Discovery-Based Non-Targeted Central Carbon Metabolism and Acyl-Carnitine Analysis via LC–MS
2.6. Co-Immunoprecipitation and MS-Based Protein Identification
2.7. Validation by Western Blot
2.8. Validation by Co-IP and Western Blot
2.9. Statistical Analysis
3. Results
3.1. Echocardiographic Phenotyping of the Studied Animals in Proteomic Studies
3.2. Visualization of the LV and RV Proteomic Datasets
3.3. Validation of the LV and RV Proteomic Findings
3.4. Effect of BNIP3 on Mitochondrial Function and Respiration in Cardiac Myocytes
3.5. Visualization and Validation of the Untargeted Metabolomic Dataset in LV Myocardia
3.6. Visualization and Validation of the LV and RV Phosphoproteomic Datasets
3.7. Visualization and Validation of BNIP3 Interactome in Rat and Human LV Myocardia
4. Discussion
4.1. BNIP3 and Remodeling of the Mt-Proteome
4.2. BNIP3 and the Regulation of ER–Mt-Calcium and Ion Homeostasis
4.3. BNIP3 and PKA, PRKCE, and AMPK Signaling
4.4. Other Effects of BNIP3
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chaanine, A.H.; Higgins, L.; Lauterboeck, L.; Markowski, T.; Yang, Q.; Delafontaine, P. Multiomics Approach Reveals an Important Role of BNIP3 in Myocardial Remodeling and the Pathogenesis of Heart Failure with Reduced Ejection Fraction. Cells 2022, 11, 1572. https://doi.org/10.3390/cells11091572
Chaanine AH, Higgins L, Lauterboeck L, Markowski T, Yang Q, Delafontaine P. Multiomics Approach Reveals an Important Role of BNIP3 in Myocardial Remodeling and the Pathogenesis of Heart Failure with Reduced Ejection Fraction. Cells. 2022; 11(9):1572. https://doi.org/10.3390/cells11091572
Chicago/Turabian StyleChaanine, Antoine H., LeeAnn Higgins, Lothar Lauterboeck, Todd Markowski, Qinglin Yang, and Patrice Delafontaine. 2022. "Multiomics Approach Reveals an Important Role of BNIP3 in Myocardial Remodeling and the Pathogenesis of Heart Failure with Reduced Ejection Fraction" Cells 11, no. 9: 1572. https://doi.org/10.3390/cells11091572