Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Culturing of Mitotic Cells
2.2. Cell Synchronization
2.3. Live-Cell Imaging
2.4. Immunofluorescence Staining and Quantification of pPLk1 Signal Intensity
2.5. Metaphase Plate Analysis
2.6. SiRNA Transfection
2.7. Western Blotting
2.8. Statistical Analysis
3. Results
3.1. Absence of Integrin-Mediated Cell Adhesion Prolongs G2 Phase and Delays Mitotic Progression in the Early Stages
3.2. Absence of Integrin-Mediated Cell Adhesion Causes Centrosome Abnormality
3.3. FAK Activity Promotes G2 to M Transition and Progression in Early Mitosis
3.4. FAK Inhibition Reduces PLK1 Activity and Thereby Impairs Centrosome Separation and Bipolar Spindle Assembly
3.5. Lack of FAK Expression Resembles the Effect of Integrin-Mediated Adhesion on Mitotic Progression in MEF Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kamranvar, S.A.; Gupta, D.K.; Wasberg, A.; Liu, L.; Roig, J.; Johansson, S. Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis. Cells 2022, 11, 1360. https://doi.org/10.3390/cells11081360
Kamranvar SA, Gupta DK, Wasberg A, Liu L, Roig J, Johansson S. Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis. Cells. 2022; 11(8):1360. https://doi.org/10.3390/cells11081360
Chicago/Turabian StyleKamranvar, Siamak A., Deepesh Kumar Gupta, Anishia Wasberg, Liangwen Liu, Joan Roig, and Staffan Johansson. 2022. "Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis" Cells 11, no. 8: 1360. https://doi.org/10.3390/cells11081360