Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Polymer–Ph Preparation
2.3. Cell Culture
2.4. Hydrogel Mechanical Properties Measurement
2.5. Cell Adhesion and Cell-Cycle Analysis
2.6. Viability Analysis
2.7. F-Actin Analysis
2.8. Statistical Analysis
3. Results
3.1. Mechanical Properties
3.2. HeLa/Fucci2 Adhesion
3.3. HeLa/Fucci2 Cell-Cycle Progression
3.4. HeLa/Fucci2 F-Actin Reorganization
3.5. NMuMG/Fucci2 Adhesion
3.6. NMuMG/Fucci2 Cell-Cycle Progression
3.7. NMuMG/Fucci2 F-Actin Reorganization
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abbreviation | Full Form |
---|---|
CLSM | Confocal laser-scanning microscope |
DMEM | Dulbecco’s Modified Eagle Medium |
DMF | N,N-dimethylformamide |
ECM | Extracellular matrix |
EGF | Epidermal growth factor |
EMT | Epithelial-to-mesenchymal transition |
FBS | Fetal bovine serum |
Fucci | Fluorescent ubiquitination-based cell-cycle indicator |
HA | Hyaluronic acid |
hASCs | Human adipose-derived stem cells |
HEPES | 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid |
HRP | Horseradish peroxidase |
LMW-HA | Low-molecular-weight hyaluronic acid |
MW | Molecular weight |
NHS | N-hydroxysuccinimide |
PBS | Phosphate buffered saline |
PFA | Paraformaldehyde |
Ph | Phenolic hydroxyl moieties |
PI | Propidium iodide |
RGD | Tripeptide arginine–glycine–aspartic acid |
RHAMM | Receptor for hyaluronic acid-mediated motility |
WSCD·HCl | Water-soluble carbodiimide hydrochloride |
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Mubarok, W.; Elvitigala, K.C.M.L.; Nakahata, M.; Kojima, M.; Sakai, S. Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels. Cells 2022, 11, 881. https://doi.org/10.3390/cells11050881
Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S. Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels. Cells. 2022; 11(5):881. https://doi.org/10.3390/cells11050881
Chicago/Turabian StyleMubarok, Wildan, Kelum Chamara Manoj Lakmal Elvitigala, Masaki Nakahata, Masaru Kojima, and Shinji Sakai. 2022. "Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels" Cells 11, no. 5: 881. https://doi.org/10.3390/cells11050881