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Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level

1
Institute of Plant Biology, Biological Research Centre, H-6726 Szeged, Hungary
2
Doctoral School of Biology, University of Szeged, H-6722 Szeged, Hungary
3
Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
4
Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, H-6720 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Current addresses: Institute for Zoology, Experimental Morphology, University of Cologne, D-50674 Cologne, Germany.
Academic Editors: Suleyman Allakhverdiev and Rajagopal Subramanyam
Cells 2022, 11(2), 285; https://doi.org/10.3390/cells11020285
Received: 28 November 2021 / Revised: 7 January 2022 / Accepted: 13 January 2022 / Published: 14 January 2022
(This article belongs to the Special Issue Research on Chlamydomonas Cell Biology)
Chlamydomonas reinhardtii is a model organism of increasing biotechnological importance, yet, the evaluation of its life cycle processes and photosynthesis on a single-cell level is largely unresolved. To facilitate the study of the relationship between morphology and photochemistry, we established microfluidics in combination with chlorophyll a fluorescence induction measurements. We developed two types of microfluidic platforms for single-cell investigations: (i) The traps of the “Tulip” device are suitable for capturing and immobilizing single cells, enabling the assessment of their photosynthesis for several hours without binding to a solid support surface. Using this “Tulip” platform, we performed high-quality non-photochemical quenching measurements and confirmed our earlier results on bulk cultures that non-photochemical quenching is higher in ascorbate-deficient mutants (Crvtc2-1) than in the wild-type. (ii) The traps of the “Pot” device were designed for capturing single cells and allowing the growth of the daughter cells within the traps. Using our most performant “Pot” device, we could demonstrate that the FV/FM parameter, an indicator of photosynthetic efficiency, varies considerably during the cell cycle. Our microfluidic devices, therefore, represent versatile platforms for the simultaneous morphological and photosynthetic investigations of C. reinhardtii on a single-cell level. View Full-Text
Keywords: cell cycle; Chlamydomonas reinhardtii; chlorophyll a fluorescence; microfluidics; non-photochemical quenching; photosynthesis; single-cell cell cycle; Chlamydomonas reinhardtii; chlorophyll a fluorescence; microfluidics; non-photochemical quenching; photosynthesis; single-cell
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MDPI and ACS Style

Széles, E.; Nagy, K.; Ábrahám, Á.; Kovács, S.; Podmaniczki, A.; Nagy, V.; Kovács, L.; Galajda, P.; Tóth, S.Z. Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level. Cells 2022, 11, 285. https://doi.org/10.3390/cells11020285

AMA Style

Széles E, Nagy K, Ábrahám Á, Kovács S, Podmaniczki A, Nagy V, Kovács L, Galajda P, Tóth SZ. Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level. Cells. 2022; 11(2):285. https://doi.org/10.3390/cells11020285

Chicago/Turabian Style

Széles, Eszter, Krisztina Nagy, Ágnes Ábrahám, Sándor Kovács, Anna Podmaniczki, Valéria Nagy, László Kovács, Péter Galajda, and Szilvia Z. Tóth. 2022. "Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level" Cells 11, no. 2: 285. https://doi.org/10.3390/cells11020285

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