Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes

Brock, Judith; Erhardt, Julia; Eisler, Stephan A.; Hörning, Marcel

doi:10.3390/cells11132122

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Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes

¹,

and

^1,*

Biobased Materials Laboratory, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, 70569 Stuttgart, Germany

Stuttgart Research Center Systems Biology (SRCSB), University of Stuttgart, 70569 Stuttgart, Germany

Author to whom correspondence should be addressed.

Academic Editor: Annette Müller-Taubenberger

Cells 2022, 11(13), 2122; https://doi.org/10.3390/cells11132122

Received: 3 June 2022 / Revised: 29 June 2022 / Accepted: 2 July 2022 / Published: 5 July 2022

(This article belongs to the Special Issue Cellular Integrity under Mechanical Stress)

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Abstract

Cells actively sense differences in topology, matrix elasticity and protein composition of the extracellular microenvironment and adapt their function and morphology. In this study, we focus on the cross-talk between matrix stiffness and protein coating density that regulates morphology and proliferation dynamics of single myocytes. For this, C2C12 myocytes were monitored on L-DOPA functionalized hydrogels of 22 different elasticity and fibronectin density compositions. Static images were recorded and statistically analyzed to determine morphological differences and to identify the optimized extracellular matrix (ECM). Using that information, selected ECMs were used to study the dynamics before and after cell proliferation by statistical comparison of distinct cell states. We observed a fibronectin-density-independent increase of the projected cell area until 12 kPa. Additionally, changes in fibronectin density led to an area that was optimum at about 2.6

μ {g / cm}^{2}

, which was confirmed by independent F-actin analysis, revealing a maximum actin-filament-to-cell-area ratio of 7.5%. Proliferation evaluation showed an opposite correlation between cell spreading duration and speed to matrix elasticity and protein density, which did not affect cell-cycle duration. In summary, we identified an optimized ECM composition and found that independent matrix properties regulate distinct cell characteristics. View Full-Text

Keywords: mechanosensitivity; proliferation; myocyte; actincytoskeleton; extracellular matrix; L-DOPA

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Brock, J.; Erhardt, J.; Eisler, S.A.; Hörning, M. Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes. Cells 2022, 11, 2122. https://doi.org/10.3390/cells11132122

AMA Style

Brock J, Erhardt J, Eisler SA, Hörning M. Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes. Cells. 2022; 11(13):2122. https://doi.org/10.3390/cells11132122

Chicago/Turabian Style

Brock, Judith, Julia Erhardt, Stephan A. Eisler, and Marcel Hörning. 2022. "Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes" Cells 11, no. 13: 2122. https://doi.org/10.3390/cells11132122

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Optimization of Mechanosensitive Cross-Talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes

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