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Article

Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis

1
cE3c—CHANGE, Departmento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1740-016 Lisboa, Portugal
2
ABC-RI, Algarve Biomedical Center Research Institute, 8005-139 Faro, Portugal
3
Faculdade de Medicina e Ciências Biomédicas (FMCB), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
4
Champalimaud Research Program, Champalimaud Center for the Unknown, 1400-038 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Present address: Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), University of Copenhagen, 2200 Copenhagen, Denmark.
These authors contributed equally to this work.
Academic Editor: Maria Mitsi
Cells 2022, 11(13), 2003; https://doi.org/10.3390/cells11132003
Received: 28 May 2022 / Revised: 15 June 2022 / Accepted: 21 June 2022 / Published: 22 June 2022
(This article belongs to the Special Issue Fibronectin in Health and Diseases 2022)
Fibronectin is essential for somite formation in the vertebrate embryo. Fibronectin matrix assembly starts as cells emerge from the primitive streak and ingress in the unsegmented presomitic mesoderm (PSM). PSM cells undergo cyclic waves of segmentation clock gene expression, followed by Notch-dependent upregulation of meso1 in the rostral PSM which induces somite cleft formation. However, the relevance of the fibronectin matrix for these molecular processes remains unknown. Here, we assessed the role of the PSM fibronectin matrix in the spatio-temporal regulation of chick embryo somitogenesis by perturbing (1) extracellular fibronectin matrix assembly, (2) integrin–fibronectin binding, (3) Rho-associated protein kinase (ROCK) activity and (4) non-muscle myosin II (NM II) function. We found that integrin–fibronectin engagement and NM II activity are required for cell polarization in the nascent somite. All treatments resulted in defective somitic clefts and significantly perturbed meso1 and segmentation clock gene expression in the PSM. Importantly, inhibition of actomyosin-mediated contractility increased the period of hairy1/hes4 oscillations from 90 to 120 min. Together, our work strongly suggests that the fibronectin–integrin–ROCK–NM II axis regulates segmentation clock dynamics and dictates the spatio-temporal localization of somitic clefts. View Full-Text
Keywords: fibronectin; fibronectin matrix assembly; actomyosin contractility; somitogenesis; segmentation clock; hairy1; cleft formation fibronectin; fibronectin matrix assembly; actomyosin contractility; somitogenesis; segmentation clock; hairy1; cleft formation
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MDPI and ACS Style

Gomes de Almeida, P.; Rifes, P.; Martins-Jesus, A.P.; Pinheiro, G.G.; Andrade, R.P.; Thorsteinsdóttir, S. Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis. Cells 2022, 11, 2003. https://doi.org/10.3390/cells11132003

AMA Style

Gomes de Almeida P, Rifes P, Martins-Jesus AP, Pinheiro GG, Andrade RP, Thorsteinsdóttir S. Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis. Cells. 2022; 11(13):2003. https://doi.org/10.3390/cells11132003

Chicago/Turabian Style

Gomes de Almeida, Patrícia, Pedro Rifes, Ana P. Martins-Jesus, Gonçalo G. Pinheiro, Raquel P. Andrade, and Sólveig Thorsteinsdóttir. 2022. "Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis" Cells 11, no. 13: 2003. https://doi.org/10.3390/cells11132003

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