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Article

Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes

by 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,* and 1,2,*
1
Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
2
Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Fulvio Reggiori
Cells 2022, 11(12), 1870; https://doi.org/10.3390/cells11121870
Received: 19 April 2022 / Revised: 23 May 2022 / Accepted: 2 June 2022 / Published: 9 June 2022
(This article belongs to the Section Cellular Metabolism)
Fatty liver, characterized by excessive lipid droplet (LD) accumulation in hepatocytes, is a common physiological condition in humans and aquaculture species. Lipid mobilization is an important strategy for modulating the number and size of cellular LDs. Cyclooxygenase (COX)-mediated arachidonic acid derivatives are known to improve lipid catabolism in fish; however, the specific derivatives remain unknown. In the present study, we showed that serum starvation induced LD degradation via autophagy, lipolysis, and mitochondrial energy production in zebrafish hepatocytes, accompanied by activation of the COX pathway. The cellular concentration of PGF2α, but not other prostaglandins, was significantly increased. Administration of a COX inhibitor or interference with PGF2α synthase abolished serum deprivation-induced LD suppression, LD–lysosome colocalization, and expression of autophagic genes. Additionally, exogenous PGF2α suppressed the accumulation of LDs, promoted the accumulation of lysosomes with LD and the autophagy marker protein LC3A/B, and augmented the expression of autophagic genes. Moreover, PGF2α enhanced mitochondrial accumulation and ATP production, and increased the transcript levels of β-oxidation- and mitochondrial respiratory chain-related genes. Collectively, these findings demonstrate that the COX pathway is implicated in lipid degradation induced by energy deprivation, and that PGF2α is a key molecule triggering autophagy, lipolysis, and mitochondrial development in zebrafish hepatocytes. View Full-Text
Keywords: arachidonic acid; ATP; cyclooxygenase; eicosanoids; lipid droplets; lipolysis; lipophagy; mitochondria; PGF2α arachidonic acid; ATP; cyclooxygenase; eicosanoids; lipid droplets; lipolysis; lipophagy; mitochondria; PGF2α
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MDPI and ACS Style

Tian, J.; Du, Y.; Yu, E.; Lei, C.; Xia, Y.; Jiang, P.; Li, H.; Zhang, K.; Li, Z.; Gong, W.; Xie, J.; Wang, G. Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes. Cells 2022, 11, 1870. https://doi.org/10.3390/cells11121870

AMA Style

Tian J, Du Y, Yu E, Lei C, Xia Y, Jiang P, Li H, Zhang K, Li Z, Gong W, Xie J, Wang G. Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes. Cells. 2022; 11(12):1870. https://doi.org/10.3390/cells11121870

Chicago/Turabian Style

Tian, Jingjing, Yihui Du, Ermeng Yu, Caixia Lei, Yun Xia, Peng Jiang, Hongyan Li, Kai Zhang, Zhifei Li, Wangbao Gong, Jun Xie, and Guangjun Wang. 2022. "Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes" Cells 11, no. 12: 1870. https://doi.org/10.3390/cells11121870

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