Insulin-like Growth Factor 2 Promotes Tissue-Specific Cell Growth, Proliferation and Survival during Development of Helicoverpa armigera
Abstract
:1. Introduction
2. Experimental Materials and Methods
2.1. Experimental Animal
2.2. Bioinformatic Analysis
2.3. Gene Cloning
2.4. Quantitative Real-Time Reverse Transcription PCR (qRT-PCR)
2.5. 20E Stimulation in Larvae
2.6. Preparation of Rabbit Polyclonal Antibody
2.7. Western Blotting
2.8. RNAi of Larvae by Feeding E. coli (HT115/DE3)
2.9. RNAi by Injection
2.10. Immunofluorescence
2.11. Statistical Analysis
3. Results
3.1. Identification of IGF-2-Like of H. armigera
3.2. IGF-2-like Was Localized in the Imaginal Midgut and in Some Fat Body Cells during Metamorphosis
3.3. Knockdown of Igf-2-like Caused Repression of the Imaginal Midgut and Fat Body Formation
4. Discussion
4.1. 20E Upregulates IGF-2-like Expression
4.2. IGF-2-like Promotes Cell Growth and Proliferation
4.3. The IGF-2-like-Positive Fat Body Cells Are the Progenitors of the Adult Fat Body
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ILP | insulin-like peptide; |
IGF | insulin-like growth factor; |
20E | 20-hydroxyecdysone; |
CASP3 | caspase 3; |
pHH3 | phosphorylated histone H3; |
HE staining | hematoxylin and eosin staining. |
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Zhao, Y.-M.; Wang, X.-P.; Jin, K.-Y.; Dong, D.-J.; Reiff, T.; Zhao, X.-F. Insulin-like Growth Factor 2 Promotes Tissue-Specific Cell Growth, Proliferation and Survival during Development of Helicoverpa armigera. Cells 2022, 11, 1799. https://doi.org/10.3390/cells11111799
Zhao Y-M, Wang X-P, Jin K-Y, Dong D-J, Reiff T, Zhao X-F. Insulin-like Growth Factor 2 Promotes Tissue-Specific Cell Growth, Proliferation and Survival during Development of Helicoverpa armigera. Cells. 2022; 11(11):1799. https://doi.org/10.3390/cells11111799
Chicago/Turabian StyleZhao, Yu-Meng, Xiao-Pei Wang, Ke-Yan Jin, Du-Juan Dong, Tobias Reiff, and Xiao-Fan Zhao. 2022. "Insulin-like Growth Factor 2 Promotes Tissue-Specific Cell Growth, Proliferation and Survival during Development of Helicoverpa armigera" Cells 11, no. 11: 1799. https://doi.org/10.3390/cells11111799