Claudin-17 Deficiency in Mice Results in Kidney Injury Due to Electrolyte Imbalance and Oxidative Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Generation and Maintenance of Cldn17−/− Mice
2.2. RNA Isolation, cDNA Preparation, and qRT-PCR
2.3. Western Blot Analysis
2.4. Analysis of Serum Electrolytes and Urine Parameters
2.5. Kidney Injury and Wet/Dry Ratio Analysis
2.6. Genomics Data Co-Expression Analyses
2.7. RNA-Seq and Bioinformatics Analysis
2.8. Ingenuity Pathway Analysis (IPA)
2.9. ROS Analysis
2.10. Analysis of Growth Hormone Expression
2.11. Statistical Analysis
3. Results
3.1. Cldn17−/− Mice Exhibit Delayed Growth
3.2. Impact of Cldn17 Loss on Blood and Urine Parameters
3.3. Cldn17−/− Mice Exhibit Hypophagia and Polydipsia
3.4. Cldn17−/− Mice Display Kidney Injury
3.5. Cldn17 Loss Affects the Expression of other Cldns in Mouse Kidneys
3.6. CLDN17 Co-Expression with Other Genes in Physiology and Pathology
3.7. RNA-Seq Analysis Revealed Alterations in Genes Involved in Inflammation, Hypoxia, and ROS Generation
3.8. Cldn17 Loss Did Not Affect the Expression of Vasopressin and Angiotensin Pathway Genes in Mice
3.9. Cldn17 Deficiency Promotes ROS Generation in the Kidneys
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Adil, M.S.; Parvathagiri, V.; Verma, A.; Liu, F.; Rudraraju, M.; Narayanan, S.P.; Somanath, P.R. Claudin-17 Deficiency in Mice Results in Kidney Injury Due to Electrolyte Imbalance and Oxidative Stress. Cells 2022, 11, 1782. https://doi.org/10.3390/cells11111782
Adil MS, Parvathagiri V, Verma A, Liu F, Rudraraju M, Narayanan SP, Somanath PR. Claudin-17 Deficiency in Mice Results in Kidney Injury Due to Electrolyte Imbalance and Oxidative Stress. Cells. 2022; 11(11):1782. https://doi.org/10.3390/cells11111782
Chicago/Turabian StyleAdil, Mir S., Varun Parvathagiri, Arti Verma, Fang Liu, Madhuri Rudraraju, S. Priya Narayanan, and Payaningal R. Somanath. 2022. "Claudin-17 Deficiency in Mice Results in Kidney Injury Due to Electrolyte Imbalance and Oxidative Stress" Cells 11, no. 11: 1782. https://doi.org/10.3390/cells11111782