Dlx5/6 Expression Levels in Mouse GABAergic Neurons Regulate Adult Parvalbumin Neuronal Density and Anxiety/Compulsive Behaviours
Abstract
:1. Introduction
2. Methods
2.1. Animals
2.2. Behavioural Tests
- −
- Open Field Test with object exploration
- −
- Marble burying test (MBT)
- −
- Nest building test
2.3. Immunohistochemistry
2.4. In Situ Hybridisation
2.5. Quantification of PV Neuronal Density
2.6. Reverse Transcription Quantitative PCR (RT-qPCR)
2.7. Statistical Analysis
3. Results
3.1. Deregulation of Dlx5/6 Expression in Mouse GABAergic Neurons
3.2. Effects of Dlx5/6 Deregulation in GABAergic Neurons on Adult PV-Interneurons Density
3.3. Behavioural Consequences of Dlx5/6 Expression Deregulation in GABAergic Neurons
- −
- Open Field Test with object exploration
- −
- Marble burying test (MBT)
- −
- Nest building test
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dlx5 | Fw: 5′ TCT CTA GGA CTGACG CAA ACA 3′ |
Rv: 5′ GTT ACA CGC CAT AGG GTC GC 3′ | |
Pgk1 | Fw: 5′ AACCTCCGCTTTCATGTAGAG 3′ |
Rv: 5′ GACATCTCCTAGTTTGGACAGTG 3′ | |
Hprt | Fw: 5′ CTCATGGACTGATTATGGACAGGAC 3′ |
Rv: 5′ GCAGGTCACCAAAGAACTTATAGCC 3′ | |
Ppia | Fw: 5′ CAACCCCACCGTGTTCTTCG 3′ |
Rv: 5′ GTGTAAAGTCCCACCCTGGC 3′ |
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Aouci, R.; El Soudany, M.; Maakoul, Z.; Fontaine, A.; Kurihara, H.; Levi, G.; Narboux-Nême, N. Dlx5/6 Expression Levels in Mouse GABAergic Neurons Regulate Adult Parvalbumin Neuronal Density and Anxiety/Compulsive Behaviours. Cells 2022, 11, 1739. https://doi.org/10.3390/cells11111739
Aouci R, El Soudany M, Maakoul Z, Fontaine A, Kurihara H, Levi G, Narboux-Nême N. Dlx5/6 Expression Levels in Mouse GABAergic Neurons Regulate Adult Parvalbumin Neuronal Density and Anxiety/Compulsive Behaviours. Cells. 2022; 11(11):1739. https://doi.org/10.3390/cells11111739
Chicago/Turabian StyleAouci, Rym, Mey El Soudany, Zakaria Maakoul, Anastasia Fontaine, Hiroki Kurihara, Giovanni Levi, and Nicolas Narboux-Nême. 2022. "Dlx5/6 Expression Levels in Mouse GABAergic Neurons Regulate Adult Parvalbumin Neuronal Density and Anxiety/Compulsive Behaviours" Cells 11, no. 11: 1739. https://doi.org/10.3390/cells11111739