Cerebral Organoids—Challenges to Establish a Brain Prototype

Eremeev, Artem V.; Lebedeva, Olga S.; Bogomiakova, Margarita E.; Lagarkova, Maria A.; Bogomazova, Alexandra N.

doi:10.3390/cells10071790

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Cerebral Organoids—Challenges to Establish a Brain Prototype

by

Artem V. Eremeev

^1,2,*

,

Olga S. Lebedeva

^1,2,

Margarita E. Bogomiakova

^1,2

,

Maria A. Lagarkova

^1,2

and

Alexandra N. Bogomazova

^1,2

¹

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia

²

Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia

^*

Author to whom correspondence should be addressed.

Cells 2021, 10(7), 1790; https://doi.org/10.3390/cells10071790

Submission received: 2 June 2021 / Revised: 6 July 2021 / Accepted: 13 July 2021 / Published: 15 July 2021

(This article belongs to the Special Issue 3D Cultures, Organoids, Organ-on-Chip: New Research and Innovation Challenges in Reproduction)

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Abstract

The new cellular models based on neural cells differentiated from induced pluripotent stem cells have greatly enhanced our understanding of human nervous system development. Highly efficient protocols for the differentiation of iPSCs into different types of neural cells have allowed the creation of 2D models of many neurodegenerative diseases and nervous system development. However, the 2D culture of neurons is an imperfect model of the 3D brain tissue architecture represented by many functionally active cell types. The development of protocols for the differentiation of iPSCs into 3D cerebral organoids made it possible to establish a cellular model closest to native human brain tissue. Cerebral organoids are equally suitable for modeling various CNS pathologies, testing pharmacologically active substances, and utilization in regenerative medicine. Meanwhile, this technology is still at the initial stage of development.

Keywords: iPSCs; brain organoids; differentiation; electrophysiology; neurotrophic factors

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MDPI and ACS Style

Eremeev, A.V.; Lebedeva, O.S.; Bogomiakova, M.E.; Lagarkova, M.A.; Bogomazova, A.N. Cerebral Organoids—Challenges to Establish a Brain Prototype. Cells 2021, 10, 1790. https://doi.org/10.3390/cells10071790

AMA Style

Eremeev AV, Lebedeva OS, Bogomiakova ME, Lagarkova MA, Bogomazova AN. Cerebral Organoids—Challenges to Establish a Brain Prototype. Cells. 2021; 10(7):1790. https://doi.org/10.3390/cells10071790

Chicago/Turabian Style

Eremeev, Artem V., Olga S. Lebedeva, Margarita E. Bogomiakova, Maria A. Lagarkova, and Alexandra N. Bogomazova. 2021. "Cerebral Organoids—Challenges to Establish a Brain Prototype" Cells 10, no. 7: 1790. https://doi.org/10.3390/cells10071790

APA Style

Eremeev, A. V., Lebedeva, O. S., Bogomiakova, M. E., Lagarkova, M. A., & Bogomazova, A. N. (2021). Cerebral Organoids—Challenges to Establish a Brain Prototype. Cells, 10(7), 1790. https://doi.org/10.3390/cells10071790

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Cerebral Organoids—Challenges to Establish a Brain Prototype

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