MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women
Abstract
:1. Introduction
2. Methods
2.1. Study Population
2.2. Plasma, Peripheral Blood Mononuclear Cells (PBMCs) and Placenta Biopsy Collection
2.3. Total RNA and miRNA Extraction from Placenta Biopsies, PBMCs and Plasma
2.4. MicroRNA Reverse Transcription and Real-Time PCR Array Analysis
2.5. Quantigene Plex Gene Expression Assay
2.6. Cytokine and Chemokine Measurement by Multiplex Assay
2.7. Statistical Analyses
3. Results
3.1. Population
3.2. MiRNA Expression in Human Plasma from SIPW and UPW
3.3. MiRNA Expression in Human Placenta Biopsies from SIPW and UPW
3.4. Gene Expression of Immune/Antiviral Selected Effectors in PBMCs and Placenta Biopsies from SIPW and UPW
3.5. Modulation of Cytokine and Chemokine Production in Plasma from SIPW and UPW
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SIPW (n = 15) | UPW (n = 6) | |
---|---|---|
Maternal baseline characteristics | ||
Maternal age, years, median (range) | 32 (21–39) | 33.5 (28–40) |
Third trimester of pregnancy, n (%) | 15 (100) | 6 (100) |
Gestational age at admission, median (range) | 39 (36–41) | 40 (38–41) |
RT-PCR assay of a maternal nasopharyngeal swab | ||
Positive, n (%) | 15 (100) | 0 (0) |
Prepregnancy BMI, kg/m2, median (range) | 23.4 (17.1–31.1) | 22.9 (18.2–33.1) |
Smoking habit, n (%) | 0 (0) | 0 (0) |
Ethnicity, Caucasian, n (%) | 9 (60) | 6 (100) |
Chronic comorbidity, n (%) | 4 (26.6) | 0 (0) |
Nulliparous n (%) | 6 (40) | 1 (16.6) |
Oxygen support without ICU admission, n (%) | 2 (13.3) | 0 (0) |
Positive chest X-ray, n (%) | 5 (33.3) | 0 (0) |
Severe case, n (%) | 2 (13.3) | 0 (0) |
Admission to ICU, n (%) | 1 (6.7) | 0 (0) |
SIPW (n = 15) | UPW (n = 6) | |
---|---|---|
Total of deliveries, n (%) | 15 (100) | 6 (100) |
Delivery mode | ||
Vaginal, n (%) | 10 (66.7) | 3 (50) |
Caesarean section, n (%) | 5 (33.3) | 3 (50) |
GA at delivery, weeks median (range) | 38 (36–40) | 40 (38–41) |
Caesarean section for severe maternal illness related to COVID-19, n (%) | 2 (13.3) | 0 (0) |
Birth weight, g, median (range) | 3160 (2665–3775) | 2955 (2715–3500) |
Umbilical artery pH, median (range) | 7.34 (7.24–7.53) | 7.31 (7.19–7.36) |
APGAR score 5′ < 7, n (%) | 1 (6.7) | 1 (16.7) |
Infected neonates, positive, n (%) | 0 (0) | NA |
NICU admission, n (%) | 0 (0) | NA |
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Saulle, I.; Garziano, M.; Fenizia, C.; Cappelletti, G.; Parisi, F.; Clerici, M.; Cetin, I.; Savasi, V.; Biasin, M. MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women. Cells 2021, 10, 1788. https://doi.org/10.3390/cells10071788
Saulle I, Garziano M, Fenizia C, Cappelletti G, Parisi F, Clerici M, Cetin I, Savasi V, Biasin M. MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women. Cells. 2021; 10(7):1788. https://doi.org/10.3390/cells10071788
Chicago/Turabian StyleSaulle, Irma, Micaela Garziano, Claudio Fenizia, Gioia Cappelletti, Francesca Parisi, Mario Clerici, Irene Cetin, Valeria Savasi, and Mara Biasin. 2021. "MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women" Cells 10, no. 7: 1788. https://doi.org/10.3390/cells10071788