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Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Department of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
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Author to whom correspondence should be addressed.
Academic Editor: Stephen Yarwood
Cells 2021, 10(3), 499; https://doi.org/10.3390/cells10030499
Received: 15 December 2020 / Revised: 11 February 2021 / Accepted: 22 February 2021 / Published: 26 February 2021
Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory. View Full-Text
Keywords: intravital microscopy; bioluminescence microscopy; immune cell imaging; imaging reporters; molecular imaging; tumor signaling; tumor–immune interactions intravital microscopy; bioluminescence microscopy; immune cell imaging; imaging reporters; molecular imaging; tumor signaling; tumor–immune interactions
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MDPI and ACS Style

Liu, T.W.; Gammon, S.T.; Piwnica-Worms, D. Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions. Cells 2021, 10, 499. https://doi.org/10.3390/cells10030499

AMA Style

Liu TW, Gammon ST, Piwnica-Worms D. Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions. Cells. 2021; 10(3):499. https://doi.org/10.3390/cells10030499

Chicago/Turabian Style

Liu, Tracy W.; Gammon, Seth T.; Piwnica-Worms, David. 2021. "Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions" Cells 10, no. 3: 499. https://doi.org/10.3390/cells10030499

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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