Nerve Growth Factor Neutralization Promotes Oligodendrogenesis by Increasing miR-219a-5p Levels
Abstract
:1. Introduction
2. Materials and Methods
2.1. Anti-NGF AD11 Mouse Model
2.2. RNA Isolation, Amplification and Labeling
2.3. Microarray Analysis
2.4. Microarray Data Analysis
2.5. RNA Isolation and Reverse Transcription-PCR
2.6. Brain Dissection and Tissue Processing
2.7. Neural Stem Cell Cultures
2.8. Oligodendrocytes Primary Cultures
2.9. NGF and Anti-NGF αD11 Antibody Treatment
2.10. Immunofluorescence on Brain Section and OLs
2.11. Design, Production and Transduction of Lentiviral Vectors
3. Results
3.1. NGF Neutralization Promotes Oligodendrogenesis and miR-219 Up-Regulation in Vivo
3.2. Oligodendrogenesis in AD4 Neurosphere Is Dependent on miR-219
3.3. Modulation of miR-219 Expression Is Dependent on NGF
3.4. Anti-NGF αD11 Neutralization of NGF Increases OL Differentiation of Rat Primary OPCs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Brandi, R.; Fabiano, M.; Giorgi, C.; Arisi, I.; La Regina, F.; Malerba, F.; Turturro, S.; Storti, A.E.; Ricevuti, F.; Amadio, S.; et al. Nerve Growth Factor Neutralization Promotes Oligodendrogenesis by Increasing miR-219a-5p Levels. Cells 2021, 10, 405. https://doi.org/10.3390/cells10020405
Brandi R, Fabiano M, Giorgi C, Arisi I, La Regina F, Malerba F, Turturro S, Storti AE, Ricevuti F, Amadio S, et al. Nerve Growth Factor Neutralization Promotes Oligodendrogenesis by Increasing miR-219a-5p Levels. Cells. 2021; 10(2):405. https://doi.org/10.3390/cells10020405
Chicago/Turabian StyleBrandi, Rossella, Marietta Fabiano, Corinna Giorgi, Ivan Arisi, Federico La Regina, Francesca Malerba, Sabrina Turturro, Andrea Ennio Storti, Flavia Ricevuti, Susanna Amadio, and et al. 2021. "Nerve Growth Factor Neutralization Promotes Oligodendrogenesis by Increasing miR-219a-5p Levels" Cells 10, no. 2: 405. https://doi.org/10.3390/cells10020405