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Cells
Volume 10
Issue 2
10.3390/cells10020241
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Article

Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

by
Magdalena Kurek
1,*,
Elisabet Åkesson
2,3,
Masahito Yoshihara
4,
Elizabeth Oliver
1,
Yanhua Cui
1,
Martin Becker
5,
João Pedro Alves-Lopes
1,†,
Ragnar Bjarnason
6,7,
Patrik Romerius
8,
Mikael Sundin
9,10,
Ulrika Norén Nyström
11,
Cecilia Langenskiöld
12,
Hartmut Vogt
13,
Lars Henningsohn
14,
Cecilia Petersen
1,
Olle Söder
1,
Jingtao Guo
15,
Rod T. Mitchell
16,17,
Kirsi Jahnukainen
1,18 and
Jan-Bernd Stukenborg
1,*
1
NORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, Sweden
2
Division of Neurogeriatrics, Department of Neurobiology Care Sciences & Society, Karolinska Institutet, 141 83 Huddinge, Sweden
3
The R & D Unit, Stockholms Sjukhem, 112 19 Stockholm, Sweden
4
Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden
5
Center of Neurodevelopmental Disorders (KIND), Department of Women’s and Children’s Health, Karolinska Institutet, Centre for Psychiatry Research, Region Stockholm and Astrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 64 Solna, Sweden
6
Children’s Medical Center, Landspítali University Hospital, 101 Reykjavik, Iceland
7
Department of Paediatrics Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland
8
Department of Paediatric Oncology and Haematology, Clinical Sciences, Lund University, Barn-och Ungdomssjukhuset Lund, Skånes Universitetssjukhus, 221 85 Lund, Sweden
9
Division of Paediatrics, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, Sweden
10
Pediatric Blood Disorders, Immunodeficiency and Stem Cell Transplantation Unit, Astrid Lindgren Children’s Hospital, Karolinska University Hospital, 141 86 Huddinge, Sweden
11
Division of Paediatrics, Department of Clinical Science, Umeå University, 901 87 Umeå, Sweden
12
Department of Paediatric Oncology, The Queen Silvia Children’s Hospital, 416 50 Gothenburg, Sweden
13
Crown Princess Victoria’s Child and Youth Hospital, and Department of Biomedical and Clinical Sciences, Linköping University, 581 83 Linköping, Sweden
14
Division of Urology, Institution for Clinical Science Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, Sweden
15
Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
16
MRC Centre for Reproductive Health, The Queen’s Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UK
17
Edinburgh Royal Hospital for Sick Children, Edinburgh EH9 1LF, UK
18
Division of Haematology-Oncology and Stem Cell Transplantation, Children’s Hospital, University of Helsinki, Helsinki University Central Hospital, 00029 Helsinki, Finland
 
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*
Authors to whom correspondence should be addressed.
†
Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QN, UK.
Academic Editor: Massimo De Felici
Cells 2021, 10(2), 241; https://doi.org/10.3390/cells10020241
Received: 3 November 2020 / Revised: 23 December 2020 / Accepted: 21 January 2021 / Published: 27 January 2021
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Abstract

Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. View Full-Text
Keywords: germ cells; basal membrane; stem cell niche; seminiferous tubules; infertility; late effects; spermatogonia; Sertoli cells germ cells; basal membrane; stem cell niche; seminiferous tubules; infertility; late effects; spermatogonia; Sertoli cells
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MDPI and ACS Style

Kurek, M.; Åkesson, E.; Yoshihara, M.; Oliver, E.; Cui, Y.; Becker, M.; Alves-Lopes, J.P.; Bjarnason, R.; Romerius, P.; Sundin, M.; Norén Nyström, U.; Langenskiöld, C.; Vogt, H.; Henningsohn, L.; Petersen, C.; Söder, O.; Guo, J.; Mitchell, R.T.; Jahnukainen, K.; Stukenborg, J.-B. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells 2021, 10, 241. https://doi.org/10.3390/cells10020241

AMA Style

Kurek M, Åkesson E, Yoshihara M, Oliver E, Cui Y, Becker M, Alves-Lopes JP, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Petersen C, Söder O, Guo J, Mitchell RT, Jahnukainen K, Stukenborg J-B. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells. 2021; 10(2):241. https://doi.org/10.3390/cells10020241

Chicago/Turabian Style

Kurek, Magdalena, Elisabet Åkesson, Masahito Yoshihara, Elizabeth Oliver, Yanhua Cui, Martin Becker, João P. Alves-Lopes, Ragnar Bjarnason, Patrik Romerius, Mikael Sundin, Ulrika Norén Nyström, Cecilia Langenskiöld, Hartmut Vogt, Lars Henningsohn, Cecilia Petersen, Olle Söder, Jingtao Guo, Rod T. Mitchell, Kirsi Jahnukainen, and Jan-Bernd Stukenborg. 2021. "Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation" Cells 10, no. 2: 241. https://doi.org/10.3390/cells10020241

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