Escherichia coli and Staphylococcus aureus Differentially Regulate Nrf2 Pathway in Bovine Mammary Epithelial Cells: Relation to Distinct Innate Immune Response
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Mastitis Pathogens
2.2. High throughout Sequencing
2.3. Real-Time Quantitative PCR
2.4. Immunoblot Analysis
2.5. Nuclear and Cytosolic Fractionation
2.6. Immunofluorescent Staining
2.7. Measurement of ROS Levels
2.8. Introduction of Small Interfering RNA against Nrf2
2.9. Determination of Cell Viability
2.10. Plasmids Construct and Transient Transfection
2.11. Statistical Analysis
3. Results
3.1. Gene Expression Profiles in E. coli- and S. aureus-Challenged pbMECs
3.2. Functional Analysis of Differentially Expressed Genes Induced by E. coli
3.3. Searching for Nrf2 Target Genes in DEGs from E. coli-Stimulated Cells
3.4. Validation of Selected Genes by qPCR
3.5. Different Response of Nrf2 Pathway to E. coli and S. aureus
3.6. Nrf2 Positively Regulates the Inflammatory Response in pbMECs
3.7. S. aureus-Induced Nrf2 Activation Impairment Is Not Due to Inadequate ROS Production
3.8. Both S. aureus and E. coli Upregulates p62/SQSTM1 Levels but Differentially Modify p62/SQSTM1 Phosphorylation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ying, Y.-T.; Yang, J.; Tan, X.; Liu, R.; Zhuang, Y.; Xu, J.-X.; Ren, W.-J. Escherichia coli and Staphylococcus aureus Differentially Regulate Nrf2 Pathway in Bovine Mammary Epithelial Cells: Relation to Distinct Innate Immune Response. Cells 2021, 10, 3426. https://doi.org/10.3390/cells10123426
Ying Y-T, Yang J, Tan X, Liu R, Zhuang Y, Xu J-X, Ren W-J. Escherichia coli and Staphylococcus aureus Differentially Regulate Nrf2 Pathway in Bovine Mammary Epithelial Cells: Relation to Distinct Innate Immune Response. Cells. 2021; 10(12):3426. https://doi.org/10.3390/cells10123426
Chicago/Turabian StyleYing, Yi-Tian, Jing Yang, Xun Tan, Rui Liu, Ying Zhuang, Jia-Xue Xu, and Wei-Jia Ren. 2021. "Escherichia coli and Staphylococcus aureus Differentially Regulate Nrf2 Pathway in Bovine Mammary Epithelial Cells: Relation to Distinct Innate Immune Response" Cells 10, no. 12: 3426. https://doi.org/10.3390/cells10123426
APA StyleYing, Y.-T., Yang, J., Tan, X., Liu, R., Zhuang, Y., Xu, J.-X., & Ren, W.-J. (2021). Escherichia coli and Staphylococcus aureus Differentially Regulate Nrf2 Pathway in Bovine Mammary Epithelial Cells: Relation to Distinct Innate Immune Response. Cells, 10(12), 3426. https://doi.org/10.3390/cells10123426