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Open AccessArticle

The Cycling of Intracellular Calcium Released in Response to Fluid Shear Stress Is Critical for Migration-Associated Actin Reorganization in Eosinophils

Department of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada
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Author to whom correspondence should be addressed.
Cells 2021, 10(1), 157; https://doi.org/10.3390/cells10010157
Received: 28 November 2020 / Revised: 7 January 2021 / Accepted: 12 January 2021 / Published: 15 January 2021
(This article belongs to the Special Issue Eosinophils beyond IL-5)
The magnitude of eosinophil mobilization into respiratory tissues drives the severity of inflammation in several airway diseases. In classical models of leukocyte extravasation, surface integrins undergo conformational switches to high-affinity states via chemokine binding activation. Recently, we learned that eosinophil integrins possess mechanosensitive properties that detect fluid shear stress, which alone was sufficient to induce activation. This mechanical stimulus triggered intracellular calcium release and hallmark migration-associated cytoskeletal reorganization including flattening for increased cell–substratum contact area and pseudopodia formation. The present study utilized confocal fluorescence microscopy to investigate the effects of pharmacological inhibitors to calcium signaling and actin polymerization pathways on shear stress-induced migration in vitro. Morphological changes (cell elongation, membrane protrusions) succeeded the calcium flux in untreated eosinophils within 2 min, suggesting that calcium signaling was upstream of actin cytoskeleton rearrangement. The inhibition of ryanodine receptors and endomembrane Ca2+-ATPases corroborated this idea, indicated by a significant increase in time between the calcium spike and actin polymerization. The impact of the temporal link is evident as the capacity of treated eosinophils to move across fibronectin-coated surfaces was significantly hampered relative to untreated eosinophils. Furthermore, we determined that the nature of cellular motility in response to fluid shear stress was nondirectional. View Full-Text
Keywords: actin; calcium signaling; confocal microscopy; integrin; mechanosensing; membrane ruffles; pseudopodia; shear stress actin; calcium signaling; confocal microscopy; integrin; mechanosensing; membrane ruffles; pseudopodia; shear stress
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MDPI and ACS Style

Son, K.; Hussain, A.; Sehmi, R.; Janssen, L. The Cycling of Intracellular Calcium Released in Response to Fluid Shear Stress Is Critical for Migration-Associated Actin Reorganization in Eosinophils. Cells 2021, 10, 157. https://doi.org/10.3390/cells10010157

AMA Style

Son K, Hussain A, Sehmi R, Janssen L. The Cycling of Intracellular Calcium Released in Response to Fluid Shear Stress Is Critical for Migration-Associated Actin Reorganization in Eosinophils. Cells. 2021; 10(1):157. https://doi.org/10.3390/cells10010157

Chicago/Turabian Style

Son, Kiho; Hussain, Amer; Sehmi, Roma; Janssen, Luke. 2021. "The Cycling of Intracellular Calcium Released in Response to Fluid Shear Stress Is Critical for Migration-Associated Actin Reorganization in Eosinophils" Cells 10, no. 1: 157. https://doi.org/10.3390/cells10010157

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