Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Growth Conditions and Measurements
2.2. Relative Water Content (RWC)
2.3. Measurement of Water-Soluble Carbohydrates (WSC)
2.4. Enzymatic Assays
2.5. Proteins Determination and Immunoblotting
2.6. RNA-Extraction and Real Time Q-RT-PCR
2.7. Bioinformatics Analyses
2.8. Statistical Analysis
3. Results
3.1. Impact of Osmotic Stress on Growth, RWC and WSC
3.2. Impact of Osmotic Stress on Enzymes Involved in Nitrogen Metabolism
3.3. Changes in Antioxidant Enzymes
3.4. Immunoblots of HSP70, PEPC, GS and Fd-GOGAT
3.5. Differential Gene Expression in Osmotic Stressed Genotypes
4. Discussion
4.1. Differential Growth Performance of Durum Wheat Genotypes under Osmotic Stress
4.2. The GS-GOGAT Pathway Is Impacted by the Osmotic Stress
4.3. Antioxidant Enzymes Activities Increased upon Osmotic Stress
4.4. Differential Biochemical Behaviors of the Three Genotypes GDH Activity and Transcript Abundance
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Height(cm) | FW (g) | DW (g) | RWC (%) | WSC (mg g−1 FW) | ||
---|---|---|---|---|---|---|
Genotypes (G) | ||||||
YT13 | 27.833 ± 1.93 | 0.361 ± 0.18 | 0.037 ± 0.007 | 80.260 ± 6.66 | 0.305 ± 0.08 | |
ON66 | 35.916 ± 4.45 | 0.418 ± 0.24 | 0.045 ± 0.01 | 85.971 ± 6.17 | 0.343 ± 0.15 | |
MAALI | 30.916 ± 1.36 | 0.372 ± 0.18 | 0.040 ± 0.006 | 82.296 ± 11.13 | 0.286 ± 0.05 | |
Treatment (T) | ||||||
Control | 32.722 ± 2.97 | 0.574 ± 0.05 | 0.049 ± 0.005 | 89.547 ± 5.57 | 0.214 ± 0.11 | |
Osmotic stress | 29.111 ± 5.2 | 0.17 ± 0.06 | 0.031 ± 0.008 | 75.046 ± 3.85 | 0.340 ± 0.1 | |
Mean | 30.916 ± 4.6 | 0.372 ± 0.21 | 0.040 ± 0.01 | 82.296 ± 8.7 | 0.286 ± 0.12 | |
ANOVA | ||||||
df | ||||||
G | 2 | 114.54 *** | 0.0105 | 0.000131 ** | 61.0 * | 0.0176 |
T | 1 | 58.68 ** | 0.7361 *** | 0.001422 *** | 946.3 *** | 0.0605 * |
G × T | 2 | 25.35 ** | 0.0070 | 0.000241 *** | 59.4 * | 0.0269 |
Error | 12 | 3.60 | 0.0029 | 0.000015 | 14.4 | 0.718 |
GS1 | GS2 | GDH | NRT2 | PEPc Activity | APX Activity | GS Activity | G6PDH | |
---|---|---|---|---|---|---|---|---|
Genotypes | ||||||||
Maali | 4.50 × 10−4 | 1.82 × 10−4 | 1.06 | 6.99 × 10−4 | 2.96 | 12.7 × 104 | 0.099 | 2.21 |
YT13 | 6.63 × 10−4 | 2.91 × 10−4 | 3.59 | 7.82 × 10−4 | 3.14 | 52.1 × 104 | 0.147 | 2.74 |
ON66 | 6.11 × 10−4 | 1.16 × 10−4 | 1.69 | 12.2 × 10−4 | 3.23 | 69.7 × 104 | 0.198 | 1.86 |
Treatments | ||||||||
Control | 8.13 × 10−4 | 3.03 × 10−4 | 1.72 | 9.26 × 10−4 | 3.69 | 12200 | 0.151 | 1.95 |
Osmotic stress | 3.36 × 10−4 | 0.89 × 10−4 | 2.56 | 8.72 × 10−4 | 2.53 | 4410 | 0.144 | 2.59 |
Means | 5.74 × 10−4 | 1.96 × 10−4 | 2.09 | 8.99 × 10−4 | 3.11 | 8280 | 0.148 | 2.27 |
ANOVA (mean square) | ||||||||
Genotype | 7.4 × 10−8 *** | 4.7 × 10−8 *** | 8.726 * | 4.6 × 10−7 *** | 11.6 | 6.07 × 107 | 1.2 × 10−2 * | 77.21 |
Treatment | 1.0 × 10−6 *** | 2.04 × 10−7 *** | 3.259 | 1.30 × 10−8 | 599 *** | 1.80 × 108 | 4.11 × 10−4 | 125.29 |
G × T | 1.9 × 10−8 * | 1.7 × 10−8 * | 23.571 ** | 1.18 × 10−8 | 14.6* | 5.86 × 107 | 9.32 × 10−4 | 5.84 |
Error | 4.7 × 10−9 | 2.6 × 10−9 | 1.697 | 9.90 × 10−9 | 3.2 | 1.22 × 108 | 15.8 × 10−4 | 144.50 |
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Jallouli, S.; Ayadi, S.; Landi, S.; Capasso, G.; Santini, G.; Chamekh, Z.; Zouari, I.; Ben Azaiez, F.E.; Trifa, Y.; Esposito, S. Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes. Agronomy 2019, 9, 550. https://doi.org/10.3390/agronomy9090550
Jallouli S, Ayadi S, Landi S, Capasso G, Santini G, Chamekh Z, Zouari I, Ben Azaiez FE, Trifa Y, Esposito S. Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes. Agronomy. 2019; 9(9):550. https://doi.org/10.3390/agronomy9090550
Chicago/Turabian StyleJallouli, Salma, Sawsen Ayadi, Simone Landi, Giorgia Capasso, Giorgia Santini, Zoubeir Chamekh, Inés Zouari, Fatma Ezzahra Ben Azaiez, Youssef Trifa, and Sergio Esposito. 2019. "Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes" Agronomy 9, no. 9: 550. https://doi.org/10.3390/agronomy9090550