Liming Positively Modulates Microbial Community Composition and Function of Sugarcane Fields
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Sample Collection
2.2. Measurement of Sucrose Content and Theoretical Yield
- (a)
- Single stalk weight (kg) = (stalk diameter (cm))2 × (stalk height (cm) − 30) × 1 (g/cm3) × 0.7854/1000.
- (b)
- Theoretical production (kg/hm2) = single stalk weight (kg) × productive stem numbers (hm2).
2.3. Measurement of Soil Chemical Properties
2.4. Soil DNA Extraction and PCR Amplification
2.5. Illumina MiSeq Sequencing
2.6. Processing and Analyzing of Sequencing Data
3. Results
3.1. Sugarcane Agronomic Properties and Production
3.2. Soil Physiochemical Properties
3.3. Microbial Alpha Diversity
3.4. Microbial Beta Diversity
3.5. Microbial Community Composition
3.6. Correlation among Soil Chemical Properties and Microbial Community
3.7. Bacterial Function Analysis
3.8. Fungal Functional Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatment | 2016 | 2017 | ||||||
---|---|---|---|---|---|---|---|---|
Urea | Calcium Superphosphate | Potassium Chloride | Quicklime | Urea | Calcium Superphosphate | Potassium Chloride | Quicklime | |
CK | 675 | 1500 | 525 | 0 | 675 | 1500 | 525 | 0 |
L1 | 675 | 1500 | 525 | 0 | 675 | 1500 | 525 | 1500 |
L2 | 675 | 1500 | 525 | 1500 | 675 | 1500 | 525 | 1500 |
Treatment | Sucrose Content (%) | Available Stalk Number (hm−2) | Stalk Height (cm) | Stalk Diameter (cm) | Single Stalk Weight (kg) | Theoretical Production (kg/hm2) |
---|---|---|---|---|---|---|
CK | 12.03 ± 0.54 a | 52,778 ± 1604 a | 300.7 ± 6.2 b | 2.56 ± 0.03 a | 1.40 ± 0.06 b | 73,716 ± 2916 a |
L1 | 12.23 ± 0.38 a | 50,926 ± 2450 a | 321.0 ± 4.6 a | 2.61 ± 0.02 a | 1.55 ± 0.04 ab | 78,974 ± 2755 a |
L2 | 12.48 ± 0.23 a | 51,852 ± 4036 a | 329.0 ± 2.3 a | 2.61 ± 0.03 a | 1.60 ± 0.03 a | 83,269 ± 7198 a |
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Pang, Z.; Tayyab, M.; Kong, C.; Hu, C.; Zhu, Z.; Wei, X.; Yuan, Z. Liming Positively Modulates Microbial Community Composition and Function of Sugarcane Fields. Agronomy 2019, 9, 808. https://doi.org/10.3390/agronomy9120808
Pang Z, Tayyab M, Kong C, Hu C, Zhu Z, Wei X, Yuan Z. Liming Positively Modulates Microbial Community Composition and Function of Sugarcane Fields. Agronomy. 2019; 9(12):808. https://doi.org/10.3390/agronomy9120808
Chicago/Turabian StylePang, Ziqin, Muhammad Tayyab, Chuibao Kong, Chaohua Hu, Zhisheng Zhu, Xin Wei, and Zhaonian Yuan. 2019. "Liming Positively Modulates Microbial Community Composition and Function of Sugarcane Fields" Agronomy 9, no. 12: 808. https://doi.org/10.3390/agronomy9120808