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Open AccessArticle

Actinobacteria Structure in Autogenic, Hydrogenic and Lithogenic Cultivated and Non-Cultivated Soils: A Culture-Independent Approach

1
Department of Biology and Biotechnology of Microorganisms, The John Paul II Catholic University of Lublin; Konstantynów St. 1 I, 20-708 Lublin, Poland
2
Department of Microbiology and Mycology, University of Warmia and Mazury in Olsztyn; Oczapowskiego St. 1a, 10-719 Olsztyn, Poland
3
Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS; Pawińskiego St. 5a, 02-206 Warsaw, Poland
4
Department of Microbial Biology, Warsaw University of Life Sciences; Nowoursynowska 159/37 St., 02-776 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Agronomy 2019, 9(10), 598; https://doi.org/10.3390/agronomy9100598
Received: 12 August 2019 / Revised: 25 September 2019 / Accepted: 27 September 2019 / Published: 29 September 2019
The aim of the study was to determine the Actinobacteria structure in cultivated (C) versus non-cultivated (NC) soils divided into three groups (autogenic, hydrogenic, lithogenic) with consideration its formation process in order to assess the Actinobacteria sensitivity to agricultural soil use and soil genesis and to identify factors affecting their abundance. Sixteen C soil samples and sixteen NC samples serving as controls were taken for the study. Next generation sequencing (NGS) of the 16S rRNA metagenomic amplicons (Ion Torrent™ technology) and Denaturing Gradient Gel Electrophoresis (DGGE) were applied for precise determination of biodiversity. Generally, greater abundance of Actinobacteria in the NC soils relative to the C soils was found. Moreover, it was indicated that the actinobacterial diversity depended on both the soil genesis and the land use; however, this effect directly depended on the particular family and genera. Two factors: redox potential (Eh) and total carbon (TC) seemed to had a significant effect on the diversity of Actinobacteria. More precisely, Actinobacteria from the NC soils displayed a greater affinity for each other and were clearly influenced by Eh, whilst those from the C soils were mostly influenced by TC. View Full-Text
Keywords: Actinobacteria; cultivated soils; wastelands; DGGE; NGS; biodiversity indices Actinobacteria; cultivated soils; wastelands; DGGE; NGS; biodiversity indices
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MDPI and ACS Style

Wolińska, A.; Górniak, D.; Zielenkiewicz, U.; Kuźniar, A.; Izak, D.; Banach, A.; Błaszczyk, M. Actinobacteria Structure in Autogenic, Hydrogenic and Lithogenic Cultivated and Non-Cultivated Soils: A Culture-Independent Approach. Agronomy 2019, 9, 598. https://doi.org/10.3390/agronomy9100598

AMA Style

Wolińska A, Górniak D, Zielenkiewicz U, Kuźniar A, Izak D, Banach A, Błaszczyk M. Actinobacteria Structure in Autogenic, Hydrogenic and Lithogenic Cultivated and Non-Cultivated Soils: A Culture-Independent Approach. Agronomy. 2019; 9(10):598. https://doi.org/10.3390/agronomy9100598

Chicago/Turabian Style

Wolińska, Agnieszka; Górniak, Dorota; Zielenkiewicz, Urszula; Kuźniar, Agnieszka; Izak, Dariusz; Banach, Artur; Błaszczyk, Mieczysław. 2019. "Actinobacteria Structure in Autogenic, Hydrogenic and Lithogenic Cultivated and Non-Cultivated Soils: A Culture-Independent Approach" Agronomy 9, no. 10: 598. https://doi.org/10.3390/agronomy9100598

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