Next Article in Journal
Genotypic Variation in Anthocyanins, Phenolic Compounds, and Antioxidant Activity in Cob and Husk of Purple Field Corn
Next Article in Special Issue
Performance Assessment of Drought Tolerant Maize Hybrids under Combined Drought and Heat Stress
Previous Article in Journal
Segmentation of Rice Seedlings Using the YCrCb Color Space and an Improved Otsu Method
Previous Article in Special Issue
Characterization for Drought Tolerance and Physiological Efficiency in Novel Cytoplasmic Male Sterile Sources of Sunflower (Helianthus annuus L.)
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Agronomy 2018, 8(11), 270; https://doi.org/10.3390/agronomy8110270

Unveiling the Enigmatic Structure of TdCMO Transcripts in Durum Wheat

1
Department of Environmental, Biological and Pharmaceutical Science and Technology, Universitàdella Campania Luigi Vanvitelli, Naples, via Vivaldi, 43, I-81100 Caserta, Italy
2
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
3
Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 2 October 2018 / Revised: 7 November 2018 / Accepted: 19 November 2018 / Published: 21 November 2018
Full-Text   |   PDF [952 KB, uploaded 21 November 2018]   |  

Abstract

Durum wheat is one of the oldest and most important edible cereal crops and its cultivation has considerable economic importance in many countries. However, adverse conditions, such as high irradiance and increasing salinity of soils, could lead to a decrease in productivity over the next few decades. Durum wheat plants under salinityare able toaccumulate glycine betaine to osmotically balance the cytosol and reduce oxidative stress, especially in young tissues. However, the synthesis of this fundamental osmolyte is inhibited by high light in T. durum even under salinity. Choline monooxygenase is the first enzyme involved in the glycine betaine biosynthetic pathway. Thus, to explain the glycine betaine inhibition, we analyzed the effect of both salinity and high light on the putative TdCMO gene expression. Thirty-eight TdCMO different transcripts were isolated in the young leaves of durum wheat grown in different stress conditions. All translated amino acid sequences, except for the TdCMO1a6 clone, showed a frame shift caused by insertions or deletions. The presence of different transcripts could depend on the presence of duplicated genes, different allelic forms, and alternative splicing events. TdCMO1a6 computational modeling of the 3D structure showed that in durum wheat, a putative CMO-like enzyme with a different Rieske type motif, is present and could be responsible for the glycine betaine synthesis. View Full-Text
Keywords: salinity; high light; choline monooxygenase gene; glycine betaine; Triticum durum L. salinity; high light; choline monooxygenase gene; glycine betaine; Triticum durum L.
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Ciarmiello, L.F.; Di Maro, A.; Woodrow, P.; Annunziata, M.G.; Kafantaris, I.; Mirto, A.; Iannuzzi, F.; Fuggi, A.; Carillo, P. Unveiling the Enigmatic Structure of TdCMO Transcripts in Durum Wheat. Agronomy 2018, 8, 270.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Agronomy EISSN 2073-4395 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top