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Agronomy 2018, 8(11), 260; https://doi.org/10.3390/agronomy8110260

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

1
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
2
USDA-ARS, Sugarcane Research Unit, Houma, LA 70360, USA
3
College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China
*
Author to whom correspondence should be addressed.
Received: 13 October 2018 / Revised: 3 November 2018 / Accepted: 7 November 2018 / Published: 12 November 2018
(This article belongs to the Special Issue Breeding and Genetics of Sugarcane and Other Sugar Crops)
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Abstract

Recently-developed molecular markers are becoming powerful tools, with applications in crop genetics and improvement. Microsatellites, or simple sequence repeats (SSRs), are widely used in genetic fingerprinting, kinship analysis, and population genetics, because of the advantages of high variability from co-dominant and multi-allelic polymorphisms, and accurate and rapid detection. However, more recent evidence suggests they may play an important role in genome evolution and provide hotspots of recombination. This review describes the development of SSR markers through different techniques, and the detection of SSR markers and applications for sugarcane genetic research and breeding, such as cultivar identification, genetic diversity, genome mapping, quantitative trait loci (QTL) analysis, paternity analysis, cross-species transferability, segregation analysis, phylogenetic relationships, and identification of wild cross hybrids. We also discuss the advantages and disadvantages of SSR markers and highlight some future perspectives. View Full-Text
Keywords: simple sequence repeats (SSRs); molecular marker; sugarcane genetic research; sugarcane breeding simple sequence repeats (SSRs); molecular marker; sugarcane genetic research; sugarcane breeding
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Ahmad, A.; Wang, J.-D.; Pan, Y.-B.; Sharif, R.; Gao, S.-J. Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies. Agronomy 2018, 8, 260.

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