Biological and Molecular Characterization of Moroccan Isolates of Spiroplasma citri
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biological Indexing Assay
2.2. Isolation and Growth of S. citri
2.3. DNA Extraction and PCR Amplification
2.4. Sequencing and Phylogenetic Analysis
3. Results
3.1. Biological INDEXING
3.2. Isolation and Culturing of S. citri
3.3. Molecular Detection
3.4. Phylogenetic Sequencing Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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No. | Sample Name | Citrus Species | Region | Isolation on R2 Liquid Medium | PCR_P58 | PCR_Spiraline | Biological Indexing | Accession Number NCBI of Sequences |
---|---|---|---|---|---|---|---|---|
1 | 1NAD | Lemon | Melouya | − | + | − | (*) | OM365903 |
2 | 3NAD | Lemon | Melouya | − | + | − | (*) | ON923969 |
3 | 2BLK | Lemon | Loukkos | + | + | − | 2 | ON939599 |
4 | 2GLK | Navel | Loukkos | − | + | + | (*) | ON923972 |
5 | 4BM | Navel | Tadla | + | + | + | 3 | OP204698 |
6 | 6TAD | Navel | Tadla | − | + | − | 2 | ON303972 |
7 | 5TAD | Navel | Tadla | + | + | + | 3 | ON303970 |
8 | 30S1 | Clementine | Tadla | + | + | + | (*) | ON303971 |
9 | 3GH | Navel | Gharb | + | + | + | 3 | OP204695 |
10 | 5GH | Navel | Gharb | − | + | − | 2 | ON236615 |
11 | 8GH | Navel | Gharb | + | + | + | (*) | OR066406 |
12 | 27GH | Clementine | Gharb | + | + | + | 1 | OP204696 |
13 | 45GH | Clementine | Gharb | + | + | − | (*) | ON303973 |
14 | 16MK | Navel | Haouz | + | + | + | 3 | OR042738 |
15 | 56MK | Navel | Haouz | + | + | + | (*) | OP204697 |
16 | 60MK | Navel | Haouz | + | + | + | 3 | OR066405 |
17 | 13SS | Navel | Souss | + | + | − | 0 | ON303968 |
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Sagouti, T.; Legrifi, I.; Rhallabi, N.; Belabess, Z.; El Jarroudi, M.; Amiri, S.; Barka, E.A.; Tahiri, A.; Lahlali, R. Biological and Molecular Characterization of Moroccan Isolates of Spiroplasma citri. Agronomy 2025, 15, 216. https://doi.org/10.3390/agronomy15010216
Sagouti T, Legrifi I, Rhallabi N, Belabess Z, El Jarroudi M, Amiri S, Barka EA, Tahiri A, Lahlali R. Biological and Molecular Characterization of Moroccan Isolates of Spiroplasma citri. Agronomy. 2025; 15(1):216. https://doi.org/10.3390/agronomy15010216
Chicago/Turabian StyleSagouti, Tourya, Ikram Legrifi, Naima Rhallabi, Zineb Belabess, Moussa El Jarroudi, Said Amiri, Essaid Ait Barka, Abdessalem Tahiri, and Rachid Lahlali. 2025. "Biological and Molecular Characterization of Moroccan Isolates of Spiroplasma citri" Agronomy 15, no. 1: 216. https://doi.org/10.3390/agronomy15010216
APA StyleSagouti, T., Legrifi, I., Rhallabi, N., Belabess, Z., El Jarroudi, M., Amiri, S., Barka, E. A., Tahiri, A., & Lahlali, R. (2025). Biological and Molecular Characterization of Moroccan Isolates of Spiroplasma citri. Agronomy, 15(1), 216. https://doi.org/10.3390/agronomy15010216