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Agronomy
Volume 12
Issue 1
10.3390/agronomy12010095
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Article

Isolation and Identification of Lipopeptide-Producing Bacillus velezensis Strains from Wheat Phyllosphere with Antifungal Activity against the Wheat Pathogen Zymoseptoria tritici

by
Rémi Platel
1,†,
Mélodie Sawicki
2,†,
Qassim Esmaeel
3,
Béatrice Randoux
2,
Pauline Trapet
1,
Mohammed El Guilli
4,
Noureddine Chtaina
5,
Ségolène Arnauld
1,6,
Alexandre Bricout
1,
Alice Rochex
1,
Natacha Bourdon
2,
Patrice Halama
1,
Cédric Jacquard
3,
Essaid Ait Barka
3,
Philippe Reignault
2,
Maryline Magnin-Robert
2,† and
Ali Siah
1,*,†
1
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
2
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
3
Unité de Recherche Résistance Induite et Bio-Protection des Plantes-EA 4707—USC INRAe1488, Université de Reims Champagne-Ardenne, 51100 Reims, France
4
Center of Agricultural Research of Kenitra 14070, Plant Pathology and Post-Harvest Quality, National Institute of Agricultural Research, Kenitra 14070, Morocco
5
Laboratory of Phytopathology, Department of Production, Protection and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat 10010, Morocco
6
Plateforme Technologique Agro-Alimentaire, Polytech Lille, Université de Lille, Cité Scientifique, 59655 Villeneuve D’Ascq, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Antonios Zambounis
Agronomy 2022, 12(1), 95; https://doi.org/10.3390/agronomy12010095
Received: 5 November 2021 / Revised: 21 December 2021 / Accepted: 26 December 2021 / Published: 31 December 2021
(This article belongs to the Special Issue Microbial Control of Crop Diseases: Limitations and Optimizations)
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Abstract

Septoria tritici blotch, caused by the fungal pathogen Zymoseptoria tritici, is a highly significant disease on wheat crops worldwide. The objective of the present study was to find out new bacterial strains with bio-antimicrobial activity against Z. tritici. Two phyllospheric bacteria (S1 and S6) were isolated from wheat ears and identified as Bacillus velezensis strains according to 16S rRNA Sanger sequencing. Antagonistic assays performed with either living strains or cell-free culture filtrates showed significant in vitro antifungal activities against Z. tritici. For the culture filtrates, the half-maximal inhibitory dilution and the minimal inhibitory dilution were 1.4% and 3.7% for the strain S1, and 7.4% and 15% for the strain S6, respectively. MALDI—ToF analysis revealed that both strains synthesize cyclic lipopeptides but from different families. Interestingly, only strain S1 produces putative bacillomycin D. Such differential lipopeptide production patterns might explain the difference observed between the antifungal activity of the culture filtrates of the two strains. This study allows the identification of new lipopeptide-producing strains of B. velezensis with a high potential of application for the biocontrol of Z. tritici. View Full-Text
Keywords: Zymoseptoria tritici; Bacillus velezensis; antimicrobial agents; cyclic lipopeptides; bacillomycin D Zymoseptoria tritici; Bacillus velezensis; antimicrobial agents; cyclic lipopeptides; bacillomycin D
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MDPI and ACS Style

Platel, R.; Sawicki, M.; Esmaeel, Q.; Randoux, B.; Trapet, P.; El Guilli, M.; Chtaina, N.; Arnauld, S.; Bricout, A.; Rochex, A.; Bourdon, N.; Halama, P.; Jacquard, C.; Barka, E.A.; Reignault, P.; Magnin-Robert, M.; Siah, A. Isolation and Identification of Lipopeptide-Producing Bacillus velezensis Strains from Wheat Phyllosphere with Antifungal Activity against the Wheat Pathogen Zymoseptoria tritici. Agronomy 2022, 12, 95. https://doi.org/10.3390/agronomy12010095

AMA Style

Platel R, Sawicki M, Esmaeel Q, Randoux B, Trapet P, El Guilli M, Chtaina N, Arnauld S, Bricout A, Rochex A, Bourdon N, Halama P, Jacquard C, Barka EA, Reignault P, Magnin-Robert M, Siah A. Isolation and Identification of Lipopeptide-Producing Bacillus velezensis Strains from Wheat Phyllosphere with Antifungal Activity against the Wheat Pathogen Zymoseptoria tritici. Agronomy. 2022; 12(1):95. https://doi.org/10.3390/agronomy12010095

Chicago/Turabian Style

Platel, Rémi, Mélodie Sawicki, Qassim Esmaeel, Béatrice Randoux, Pauline Trapet, Mohammed El Guilli, Noureddine Chtaina, Ségolène Arnauld, Alexandre Bricout, Alice Rochex, Natacha Bourdon, Patrice Halama, Cédric Jacquard, Essaid A. Barka, Philippe Reignault, Maryline Magnin-Robert, and Ali Siah. 2022. "Isolation and Identification of Lipopeptide-Producing Bacillus velezensis Strains from Wheat Phyllosphere with Antifungal Activity against the Wheat Pathogen Zymoseptoria tritici" Agronomy 12, no. 1: 95. https://doi.org/10.3390/agronomy12010095

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