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Article

The Role of Laccase from Zygomycetous Fungus Mortierella elasson in Humic Acids Degradation

1
Federal Research Center “Puschino Scientific Center for Biological Research of the Russian Academy of Sciences”, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, 142290 Pushchino, Russia
2
Moscow State University, Faculty of Soil Science, Faculty of Chemistry, 119992 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editors: Evgeny Lodygin, Evgeny Abakumov and Elena Shamrikova
Agronomy 2021, 11(11), 2169; https://doi.org/10.3390/agronomy11112169
Received: 30 September 2021 / Revised: 25 October 2021 / Accepted: 26 October 2021 / Published: 28 October 2021
Biochemical stability of soil humus is an important factor affecting soil quality. Fungi are among the most efficient decomposers of humic matter due to presence of oxidative enzymes, including phenoloxidase laccase. Production of laccase by zygomycetes, a group of cellulolytic fungi widespread in soil, is poorly studied. The potential role of laccase from zygomycetes in humus turnover is unknown. Here, we show for the first time that laccase of zygomycetous fungus Mortierella elasson can effectively depolymerize humic acids in vitro. The fungus produced laccase extracellularly in a liquid culture medium. Unlike in case of laccases in ligninolytic basidiomycetes, attempts to increase enzyme activity using inductors, changes in the source of nitrogen and carbon failed to lead to any increase in laccase production. Laccase was purified using ion exchange chromatography and gel filtration. The molecular weight of the laccase was 51.75 kDa. The laccase catalyzed the oxidation of ABTS and K4[Fe(CN)6], phenolic compounds, but not tyrosine. The laccase activity was inhibited by NaN3 and NaF. The pH optimum of the laccase activity was 3.0 for ABTS and 5.0 for 2,6-dimethoxy phenol. The enzyme had moderate thermal stability and was rapidly inactivated at 70 °C. Purified laccase depolymerized humic acids from retisol, compost and peat more effectively than culture liquid containing laccase. The results of the study extend our knowledge of the role of laccases from different producers in the transformation of natural organic matter. View Full-Text
Keywords: soil organic matter; decomposition; humic substances; laccase; cellulolytic fungi soil organic matter; decomposition; humic substances; laccase; cellulolytic fungi
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MDPI and ACS Style

Lisov, A.; Belova, O.; Zavarzina, A.; Konstantinov, A.; Leontievsky, A. The Role of Laccase from Zygomycetous Fungus Mortierella elasson in Humic Acids Degradation. Agronomy 2021, 11, 2169. https://doi.org/10.3390/agronomy11112169

AMA Style

Lisov A, Belova O, Zavarzina A, Konstantinov A, Leontievsky A. The Role of Laccase from Zygomycetous Fungus Mortierella elasson in Humic Acids Degradation. Agronomy. 2021; 11(11):2169. https://doi.org/10.3390/agronomy11112169

Chicago/Turabian Style

Lisov, Alexander, Oksana Belova, Anna Zavarzina, Andrey Konstantinov, and Alexey Leontievsky. 2021. "The Role of Laccase from Zygomycetous Fungus Mortierella elasson in Humic Acids Degradation" Agronomy 11, no. 11: 2169. https://doi.org/10.3390/agronomy11112169

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