Development and Characteristics of Interspecific Hybrids between Brassica oleracea L. and B. napus L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Interspecific Hybridization B. oleracea × B. napus
2.2. Embryo Rescue
2.3. Analysis of the Morphological Characteristics
2.4. Nuclear DNA Content
2.5. Pollen Grain Length and Viability
2.6. Chromosome Preparation
2.7. Fluorescence In Situ Hybridization
2.8. Statistical Analyses
3. Results
3.1. Interspecific Hybridization
3.2. Morphological Characteristics
3.3. Nuclear DNA Contents
3.4. Pollen Characteristics
3.5. Cytology and Physical Mapping of 5S and 35S rDNA Loci in Parental Genotypes and Interspecific Hybrids of B. oleracea × B. napus Using FISH
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Genotype | Avg. Length of Pollen (µm) * | Std. Dev. | Malformed and Undeveloped Grains (%) |
---|---|---|---|
Parental lines | |||
P1162 ♂ rapeseed | 26.63 f | 1.90 | 0.00 |
P18 ♀ Brussels sprout | 25.49 f | 0.83 | 10.99 |
J13 ♀ Brussels sprout | 21.33 g | 2.40 | 5.26 |
ZHG02 ♀ kale | 24.79 g–f | 1.12 | 4.34 |
ZGH08 ♀ kale | 25.51 g–f | 2.01 | 2.73 |
T02009 ♀ B. taurica | 27.01 f | 2.87 | 7.24 |
KA251 ♀ head cabbage | 24.94 g–f | 1.23 | 3.00 |
IW1018 ♀ head cabbage | 24.90 g–f | 2.89 | 11.50 |
Interspecific hybrids of the F1 generation | |||
S1 F1 kale × rapeseed | 38.06 a | 3.15 | 79.32 |
S2 F1 kale × rapeseed | 36.13 a–d | 4.53 | 84.72 |
S15 F1 kale × rapeseed | 36.43 a–d | 4.44 | 88.39 |
S20 F1 kale × rapeseed | 36.60 a–c | 4.43 | 86.00 |
X22 F1 kale × rapeseed | 37.71 a | 4.44 | 90.10 |
S9 F1 B. taurica × rapeseed | 35.73 a–d | 1.70 | 81.71 |
S7 F1 head cabbage × rapeseed | 37.50 a | 3.93 | 93.12 |
S14 F1 Brussels sprout × rapeseed | 39.64 a | 4.01 | 77.49 |
S18 F1 Brussels sprout × rapeseed | 38.10 a | 4.78 | 90.40 |
Interspecific hybrids of the F2 generation | |||
S14 F1 × S14 F1 | 34.72 a–c | 3.13 | 7.21 |
S15 F1 × S2 F1 | 33.20 b–c | 1.35 | 11.36 |
Interspecific hybrids of the F1 × F2 generation | |||
S1F1 × (S15 F1 × S2 F1) | 34.64 a–e | 3,40 | 35.79 |
S7 F1 × (S14 F1 × S14 F1) | 37.60 a | 3.15 | 45.30 |
S7 F1 × (S15 F1 ×S2 F1) | 37.74 b–e | 3.20 | 48.59 |
S20 F1 × (S14 F1 × S14 F1) | 32.93 d–e | 3.56 | 42.50 |
Genotype | Chromosome Number | 5S rDNA | 35S rDNA | Marker Chromosomes | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Genome C | Genome A | |||||||||
C4 a | C8 | C7 | A1 | A3 | A10 | A5/A6/A9 | ||||
Parental lines | ||||||||||
PN1162 rapeseed | 38 | 8 | 12 | 2 | 2 | 2 | 2 | 2 | 2 | 4 |
T02009 B. taurica | 18 | 2 | 6 | 2 | 2 | 2 | ||||
ZHG08 kale | 18 | 2 | 4 | 2 | 2 | 2 | ||||
ZGH02 kale | 18 | 2 | 4 | 2 | 2 | 2 | ||||
IW1018 head cabbage | 18 | 2 | 4 | 2 | 2 | 2 | ||||
KA251 head cabbage | 18 | 2 | 4 | 2 | 2 | 2 | ||||
P18 Brussels sprout | 18 | 2 | 4 | 2 | 2 | 2 | ||||
Interspecific hybrids of the F1generation | ||||||||||
S1 F1 kale × rapeseed | 27 | 4–5 | 8 | 2 | 2 | 3 | 1 | 1 | 1 | 1 |
S2 F1 kale × rapeseed | 28 | 5 | 8 | 2 | 2 | 2 | 1 | 1 | 1 | 3 |
S7 F1 head cabbage × rapeseed | 28 | 6 | 8 | 2 | 2 | 2 | 1 | 1 | 1 | 1 |
S14 F1 Brussels sprout × rapeseed | 28 | 6 | 8 | 2 | 2 | 2 | 1 | 2 | 1 | 1 |
S15 F1 kale × rapeseed | 29 | 7 | 8 | 2 | 2 | 2 | 1 | 3 | 1 | 1 |
S18 F1 Brussels sprout × rapeseed | 29 | 8 | 9 | 3 | 2 | 2 | 2 | 2 | 1 | 1 |
S20 F1 kale × rapeseed | 27 | 5 | 6 | 3 | 2 | 2 | 1 | 1 | 1 | 1 |
Interspecific hybrids of the F2 generations | ||||||||||
S14 F1 × S14 F1 | 52 | 8 | 13 | 2 | 2 | 1 | 1 | 1 | 1 | 1 |
S15 F1 × S2 F1 | 56 | 13 | 13 | 2 | 2 | 2 | 5 | 2 | 4 | 2 |
Interspecific hybrids of the BC1 generation (head cabbage × F1/F2) | ||||||||||
CPS8 × (S15 F1 × S2 F1) | 37,38 | 4-5 | 6 | 2 | 2 | 2 | 1 | 1 | ||
KLG × S7 F1 | 28, | 3 | 8 | 2 | 3 | 3 | 1 | 1 | ||
KLG × (S14 F1 × S14 F1) | 34,36 | 5 | 9 | 3 | 2 | 3 | 1 | 1 | 3 | |
TKL × (S15 F1 × S2 F1)/1 | 38 | 4 | 9 | 1 | 1 | 3 | 1 | 2 | ||
TKL × (S15 F1 × S2 F1)/2 | 28 | 5 | 8 | 2 | 2 | 1 | 1 | 1 | 1 | |
Interspecific hybrids of the BC1× (F1 × F2) generation | ||||||||||
(KLG × S7) × (S1 × S14)/1 | 39,40 | 7 | 14 | 3 | 6 | 4 | 1 | 2 | 4 | |
(KLG × S7) × (S1 × S14)/2 | 46 | 6 | 13 | 2 | 3 | 3 | 1 | 1 | 2 | |
[(CPS8× (S15 ×S2)] × S14 /1 | 43 | 4–5 | 12–14 | 1–2 | 4 | 4 | 2 | 1 | 1 | |
[(CPS8 × (S15 ×S2)] × S14/2 | 38,39 | 8 | 12 | 2 | 4 | 3 | 1 | 2 | 1 | 4 |
[(CPS8×(S15×S2)] × S1 × (S15×S2)/1 | 39 | 5 | 11 | 1–2 | 2 | 2 | 1 | 1 | 5 |
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Kamiński, P.; Marasek-Ciolakowska, A.; Podwyszyńska, M.; Starzycki, M.; Starzycka-Korbas, E.; Nowak, K. Development and Characteristics of Interspecific Hybrids between Brassica oleracea L. and B. napus L. Agronomy 2020, 10, 1339. https://doi.org/10.3390/agronomy10091339
Kamiński P, Marasek-Ciolakowska A, Podwyszyńska M, Starzycki M, Starzycka-Korbas E, Nowak K. Development and Characteristics of Interspecific Hybrids between Brassica oleracea L. and B. napus L. Agronomy. 2020; 10(9):1339. https://doi.org/10.3390/agronomy10091339
Chicago/Turabian StyleKamiński, Piotr, Agnieszka Marasek-Ciolakowska, Małgorzata Podwyszyńska, Michał Starzycki, Elżbieta Starzycka-Korbas, and Katarzyna Nowak. 2020. "Development and Characteristics of Interspecific Hybrids between Brassica oleracea L. and B. napus L." Agronomy 10, no. 9: 1339. https://doi.org/10.3390/agronomy10091339