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Open AccessArticle

In Vitro Regeneration, Ex Vitro Rooting and Foliar Stoma Studies of Pseudostellaria heterophylla (Miq.) Pax

School of Life Sciences, Qufu Normal University, Qufu 273165, China
Shandong Drug and Food Vocational College, Weihai 264210, China
Department of Horticulture, Division of Applied Life Science (BK21 Plus Program), Graduate School of Gyeongsang National University, Jinju 52828, Korea
Author to whom correspondence should be addressed.
Agronomy 2020, 10(7), 949;
Received: 4 June 2020 / Revised: 19 June 2020 / Accepted: 28 June 2020 / Published: 1 July 2020
(This article belongs to the Special Issue Role of Plant Tissue Culture in Agricultural Research and Production)
Pseudostellaria heterophylla, in the family Caryophyllaceae, is an important Chinese medicinal plant commonly used to treat various diseases in children and valued for its ornamental properties. In this study, nodal segments were obtained from wild plants and used as explants to develop an efficient micropropagation protocol for this species. Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 6-benzyladenine (6-BA) was the most suitable medium for inducing axillary buds and enhancing their growth, and MS medium containing 0.1 mg·L−1 indole-3-butyric acid (IBA) was the most effective for inducing in vitro rooting. To reduce labor, time, and cost, microshoots were rooted under ex vitro conditions. Pretreatments of the shoots with 100 mg·L−1 naphthaleneacetic acid (NAA) for 1 min ensured successful rooting in 86.7% of shoots. Comparison of the leaf microstructure between in vitro- and ex vitro-rooted plantlets revealed abnormal stomatal apparatus in the former. The stomatal apparatus of ex vitro plantlets were normal, although the stomatal density was reduced, which indicated that these plantlets were more likely to be able to adapt to environmental conditions in the field. We identified the optimal medium for P. heterophylla multiplication with respect to increased rooting efficiency of micropropagated shoots under ex vitro conditions. This results presented here will be helpful for agricultural cultivation of P. heterophylla.
Keywords: plant tissue culture; microshoots; rooting; stoma plant tissue culture; microshoots; rooting; stoma
MDPI and ACS Style

Wang, F.; Xin, X.; Wei, H.; Qiu, X.; Liu, B. In Vitro Regeneration, Ex Vitro Rooting and Foliar Stoma Studies of Pseudostellaria heterophylla (Miq.) Pax. Agronomy 2020, 10, 949.

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