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Article

Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation

1
School of Life and Environmental Sciences, Faculty of Science, The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW 2570, Australia
2
Agriculture Victoria, Centre for AgriBioscience, AgriBio, 5 Ring Road, Bundoora, VIC 3083, Australia
3
School of Applied Systems Biology, La Trobe University, Bundoora, VIC 3086, Australia
4
CSIRO Agriculture Flagship, GPO Box1600, Canberra, ACT 2601, Australia
*
Authors to whom correspondence should be addressed.
Current address: Department of Agricultural Biology, The University of Jaffna, Aryviyal Nagar, Kilinochchi 44000, Sri Lanka.
These authors contributed equally to this work.
Agronomy 2020, 10(7), 943; https://doi.org/10.3390/agronomy10070943
Received: 27 April 2020 / Revised: 10 June 2020 / Accepted: 20 June 2020 / Published: 1 July 2020
(This article belongs to the Section Pest and Disease Management)
Among the rust diseases, leaf rust of wheat caused by Puccinia triticina, is the most prevalent worldwide and causes significant yield losses. This study aimed to determine the genomic location of loci that control adult plant resistance (APR) to leaf rust in the pre-Green Revolution landrace accession, Aus27506, from the “Watkins Collection”. An Aus27506/Aus27229-derived F7 recombinant inbred line (RIL) population was screened under field conditions across three cropping seasons and genotyped with the iSelect 90K Infinium SNP bead chip array. One quantitative trait loci (QTL) on each of the chromosomes 1BL, 2B and 2DL explained most of the leaf rust response variation in the RIL population, and these were named QLr.sun-1BL, QLr.sun-2B and QLr.sun-2DL, respectively. QLr.sun-1BL and QLr.sun-2DL were contributed by Aus27506. QLr.sun-1BL is likely Lr46, while QLr.sun-2DL appeared to be a new APR locus. The alternate parent, Aus27229, carried the putatively new APR locus QLr.sun-2B. The comparison of average severities among RILs carrying these QTL in different combinations indicated that QLr.sun-2B does not interact with either of the other two QTL; however, the combination of QLr.sun-1BL and QLr.sun-2DL reduced disease severity significantly. In planta fungal quantification assays validated these results. The RILs carrying QLr.sun-1BL and QLr.sun-2DL did not differ significantly from the parent Aus27506 in terms of resistance. Aus27506 can be used as a source of adult plant leaf rust resistance in breeding programs. View Full-Text
Keywords: wheat; leaf rust; QTL mapping wheat; leaf rust; QTL mapping
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MDPI and ACS Style

Kandiah, P.; Chhetri, M.; Hayden, M.; Ayliffe, M.; Bariana, H.; Bansal, U. Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation. Agronomy 2020, 10, 943. https://doi.org/10.3390/agronomy10070943

AMA Style

Kandiah P, Chhetri M, Hayden M, Ayliffe M, Bariana H, Bansal U. Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation. Agronomy. 2020; 10(7):943. https://doi.org/10.3390/agronomy10070943

Chicago/Turabian Style

Kandiah, Pakeerathan; Chhetri, Mumta; Hayden, Matthew; Ayliffe, Michael; Bariana, Harbans; Bansal, Urmil. 2020. "Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation" Agronomy 10, no. 7: 943. https://doi.org/10.3390/agronomy10070943

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